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https://doi.org/10.1016/j.dci.2023.105124
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Garcia Alvarez, M. A. | - |
dc.contributor.author | Chaves Pozo, E. | - |
dc.contributor.author | Cuesta Peñafiel, A. | - |
dc.date.accessioned | 2024-02-14T23:11:32Z | - |
dc.date.available | 2024-02-14T23:11:32Z | - |
dc.date.issued | 2024-03 | - |
dc.identifier.citation | Developmental & Comparative Immunology Volume 152, March 2024, 105124 | es_ES |
dc.identifier.issn | 0145-305X | - |
dc.identifier.uri | http://hdl.handle.net/10201/139389 | - |
dc.description | © 2023. The authors. This document is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc /4.0/ This document is the accepted version of a published work that appeared in final form in Developmental and Comparative Immunology | - |
dc.description.abstract | Cell-mediated cytotoxicity (CMC) is essential in eradicating virus-infected cells, involving CD8+ T lymphocytes (CTLs) and natural killer (NK) cells, through the activation of different pathways. This immune response is well-studied in mammals but scarcely in teleost fish. Our aim was to investigate the adaptive CMC using head-kidney (HK) cells from European sea bass infected at different times with nodavirus (NNV), as effector cells, and the European sea bass brain cell line (DLB-1) infected with different NNV genotypes, as target cells. Results showed low and unaltered innate cytotoxic activity through the infection time. However, adaptive CMC against RGNNV and SJNNV/RGNNV-infected target cells increased from 7 to 30 days post-infection, peaking at 15 days, demonstrating the specificity of the cytotoxic activity and suggesting the involvement of CTLs. At transcriptomic level, we observed up-regulation of genes related to T cell activation, perforin/granzyme and Fas/FasL effector pathways as well as apoptotic cell death. Further studies are necessary to understand the adaptive role of European sea bass CTLs in the elimination of NNV-infected cells. | - |
dc.format | application/pdf | es_ES |
dc.language | eng | es_ES |
dc.publisher | Elsevier | - |
dc.relation.isreferencedby | ED_IDENTRADA=1351 | - |
dc.rights | info:eu-repo/semantics/openAccess | - |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject | Cytotoxic T lymphocytes (CTLs) | - |
dc.subject | Perforin | - |
dc.subject | Granzyme | - |
dc.subject | Nodavirus | - |
dc.subject | DLB-1 | - |
dc.subject.other | European sea bass | - |
dc.title | Cytotoxic activity and gene expression during in vitro adaptive cell-mediated cytotoxicity of head-kidney cells from betanodavirus-infected European sea bass | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0145305X23012776?via%3Dihub | - |
dc.identifier.doi | https://doi.org/10.1016/j.dci.2023.105124 | - |
Aparece en las colecciones: | Artículos: Biología Celular e Histología |
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2024NNVCMCDCI.pdf | 736,63 kB | Adobe PDF | ![]() Visualizar/Abrir |
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