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https://doi.org/10.1515/cclm-2021-0324
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Franco Martínez, Lorena | - |
dc.contributor.author | Tecles, Fernando | - |
dc.contributor.author | Torres Cantero, Alberto | - |
dc.contributor.author | Bernal, Enrique | - |
dc.contributor.author | San Lázaro, Indra | - |
dc.contributor.author | Alcaraz, María José | - |
dc.contributor.author | Vicente Romero, Rosario | - |
dc.contributor.author | Lamy, Elsa | - |
dc.contributor.author | Sánchez Resalt, Cristina | - |
dc.contributor.author | Rubio, Camila P. | - |
dc.contributor.author | Tvarijonaviciute, Asta | - |
dc.contributor.author | Martínez Subiela, Silvia | - |
dc.contributor.author | Cerón, José J. | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU:: Medicina y Cirugía Animal | - |
dc.date.accessioned | 2024-02-09T09:36:11Z | - |
dc.date.available | 2024-02-09T09:36:11Z | - |
dc.date.issued | 2021-04-28 | - |
dc.identifier.citation | Clinical Chemistry and Laboratory Medicine (CCLM) 59( 9) (2021): 1592–1599 | - |
dc.identifier.issn | Print: 1434-6621 | - |
dc.identifier.issn | Electronic: 1437-4331 | - |
dc.identifier.uri | http://hdl.handle.net/10201/139072 | - |
dc.description | ©2021. This document is the published, version of a Published Work that appeared in final form in Clinical Chemistry and Laboratory Medicine (CCLM). To access the final edited and published work see https://doi.org/10.1515/cclm-2021-0324 | - |
dc.description.abstract | Objectives: The aim of the present study was to validate a commercially available automated assay for the measurement of total adenosine deaminase (tADA) and its isoenzymes (ADA1 and ADA2) in saliva in a fast and accurate way, and evaluate the possible changes of these analytes in individuals with SARS-CoV-2 infection. Methods: The validation, in addition to the evaluation of precision and accuracy, included the analysis of the effects of the main procedures that are currently being used for SARS-CoV-2 inactivation in saliva and a pilot study to evaluate the possible changes in salivary tADA and isoenzymes in individuals infected with SARS-CoV-2. Results: The automated assay proved to be accurate and precise, with intra- and inter-assay coefficients of variation below 8.2%, linearity under dilution linear regression with R2 close to 1, and recovery percentage between 80 and 120% in all cases. This assay was affected when the sample is treated with heat or SDS for virus inactivation but tolerated Triton X-100 and NP-40. Individuals with SARS-CoV-2 infection (n=71) and who recovered from infection (n=11) had higher mean values of activity of tADA and its isoenzymes than healthy individuals (n=35). Conclusions: tADA and its isoenzymes ADA1 and ADA2 can be measured accurately and precisely in saliva samples in a rapid, economical, and reproducible way and can be analyzed after chemical inactivation with Triton X-100 and NP-40. Besides, the changes observed in tADA and isoenzymes in individuals with COVID-19 open the possibility of their potential use as non-invasive biomarkers in this disease. | es |
dc.format | application/pdf | es |
dc.format.extent | 8 | - |
dc.language | eng | es |
dc.relation | Seneca Foundation 19894/GERM/15 Portuguese Science Foundation CEECIND/04397/2017 | es |
dc.rights | info:eu-repo/semantics/embargoedAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.subject | Analytical validation | es |
dc.subject | Assay | es |
dc.subject | Biomarkers | es |
dc.subject | COVID-19 | es |
dc.subject | SARS-CoV-2 | es |
dc.title | Analytical validation of an automated assay for the measurement of adenosine deaminase (ADA) and its isoenzymes in saliva and a pilot evaluation of their changes in patients with SARS-CoV-2 infection | es |
dc.type | info:eu-repo/semantics/article | es |
dc.relation.publisherversion | https://www.degruyter.com/document/doi/10.1515/cclm-2021-0324/html | es |
dc.embargo.terms | Si | - |
dc.identifier.doi | https://doi.org/10.1515/cclm-2021-0324 | - |
Aparece en las colecciones: | Artículos: Medicina y Cirugía Animal |
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