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Título: | Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster. |
Fecha de publicación: | 18-feb-2016 |
Editorial: | MDPI |
Cita bibliográfica: | International Journal of Molecular Sciences, 17 (2), 2016, 251 |
ISSN: | 1661-6596 1422-0067 (electrónico) |
Palabras clave: | Brassicaceae Sulforaphene Radish Spargel Energy metabolism |
Resumen: | We used Drosophila melanogaster as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (Raphanus sativus cv. Rambo), a Brassicaceae species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/L) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that Drosophila melanogaster takes up and metabolizes isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of spargel expression, the Drosophila homolog of the mammalian PPARγ-coactivator 1 α, as well as the inhibition of α-amylase and α-glucosidase in vitro. Overall, we show that the consumption of radish sprouts affects energy metabolism in Drosophila melanogaster which is reflected by lower glucose levels and an increased expression of spargel, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellitus |
Autor/es principal/es: | Baenas, Nieves Piegholdt, Stefanie Schloesser, Anke Moreno, Diego A. García-Viguera, Cristina Rimbach, Gerald Wagner, Anika E. |
Facultad/Departamentos/Servicios: | Department of Food Science and Technology, CEBAS-CSIC |
Versión del editor: | https://www.mdpi.com/1422-0067/17/2/251 |
URI: | http://hdl.handle.net/10201/138165 |
DOI: | https://doi.org/10.3390/ijms17020251 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 10 |
Derechos: | info:eu-repo/semantics/openAccess Atribución 4.0 Internacional |
Descripción: | Item en revisión. Pendiente de cumplimentar metadatos. ©<2017>. This manuscript version is made available under the CC-BY license http://creativecommons.org/licenses/cc-by /4.0/ This document is the Published version of a Published Work that appeared in final form in [International Journal of Molecular Sciences]. To access the final edited and published work see [https://doi.org/10.3390/ijms17020251] |
Aparece en las colecciones: | Artículos: Tecnología de Alimentos, Nutrición y Bromatología |
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2016_ Baenas Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster.pdf | IJMS open access | 880,9 kB | Adobe PDF | Visualizar/Abrir |
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