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Título: | Solubilization and characterization of a cell wall-bound trehalase from ascospores of the fission yeast Schizosaccharomyces pombe |
Fecha de publicación: | 2009 |
Editorial: | Jena : G. Fischer |
Cita bibliográfica: | Microbiological Research Vol. 164(3): 304-311. 2009 |
ISSN: | 0944-5013 1618-0623 |
Materias relacionadas: | CDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiología CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica |
Palabras clave: | fission yeast trehalase microbiology cell wall ascospore |
Resumen: | The genome of the fission yeast Schizosaccharomyces pombe lacks sequence homologs to ath1 genes coding for acid trehalases in other yeasts or filamentous fungi. However, acid trehalase activity is present at the spore stage in the life cycle of the fission yeast. The enzyme responsible for this activity behaves as a surface enzyme covalently linked to the spore cell walls in both wild-type and ntp1 mutant strains devoid of neutral trehalase. Lytic treatment of particulated cell wall fractions allowed the solubilization of the enzyme into an active form. We have characterized this soluble enzyme and found that its kinetic parameters, optimum pH and temperature, thermal denaturation and salt responses are closely similar to other conventional acid trehalases. Hence, this rather unusual enzyme can be recognized as acid trehalase by its biochemical properties although it does not share genetic homology with other known acid trehalases. The potential role of such acid trehalase in the mobilization of trehalose is discussed. |
Autor/es principal/es: | Vicente, Jero Soto, Teresa Madrid, Marisa Núñez, Andrés Cansado, José Gacto, Mariano |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Genética y Microbiología |
Versión del editor: | https://www.sciencedirect.com/science/article/pii/S0944501307000419 |
URI: | http://hdl.handle.net/10201/137936 |
DOI: | https://doi.org/10.1016/j.micres.2006.12.005 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 8 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Descripción: | ©<2009>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published Manuscript version of a Published Work that appeared in final form in [Microbiological Research]. To access the final edited and published work see [https://doi.org/10.1016/j.micres.2006.12.005] |
Aparece en las colecciones: | Artículos: Genética y Microbiología |
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