Por favor, use este identificador para citar o enlazar este ítem:
https://doi.org/10.1016/j.micres.2006.12.005
Twittear
Registro completo de metadatos
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Vicente, Jero | - |
dc.contributor.author | Soto, Teresa | - |
dc.contributor.author | Madrid, Marisa | - |
dc.contributor.author | Núñez, Andrés | - |
dc.contributor.author | Cansado, José | - |
dc.contributor.author | Gacto, Mariano | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Genética y Microbiología | es |
dc.date.accessioned | 2024-01-29T11:35:19Z | - |
dc.date.available | 2024-01-29T11:35:19Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Microbiological Research Vol. 164(3): 304-311. 2009 | es |
dc.identifier.issn | 0944-5013 | - |
dc.identifier.issn | 1618-0623 | - |
dc.identifier.uri | http://hdl.handle.net/10201/137936 | - |
dc.description | ©<2009>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published Manuscript version of a Published Work that appeared in final form in [Microbiological Research]. To access the final edited and published work see [https://doi.org/10.1016/j.micres.2006.12.005] | es |
dc.description.abstract | The genome of the fission yeast Schizosaccharomyces pombe lacks sequence homologs to ath1 genes coding for acid trehalases in other yeasts or filamentous fungi. However, acid trehalase activity is present at the spore stage in the life cycle of the fission yeast. The enzyme responsible for this activity behaves as a surface enzyme covalently linked to the spore cell walls in both wild-type and ntp1 mutant strains devoid of neutral trehalase. Lytic treatment of particulated cell wall fractions allowed the solubilization of the enzyme into an active form. We have characterized this soluble enzyme and found that its kinetic parameters, optimum pH and temperature, thermal denaturation and salt responses are closely similar to other conventional acid trehalases. Hence, this rather unusual enzyme can be recognized as acid trehalase by its biochemical properties although it does not share genetic homology with other known acid trehalases. The potential role of such acid trehalase in the mobilization of trehalose is discussed. | es |
dc.format | application/pdf | es |
dc.format.extent | 8 | es |
dc.language | eng | es |
dc.publisher | Jena : G. Fischer | es |
dc.relation | Sin financiación externa a la Universidad | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | fission yeast | es |
dc.subject | trehalase | es |
dc.subject | microbiology | es |
dc.subject | cell wall | es |
dc.subject | ascospore | es |
dc.subject.other | CDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiología | es |
dc.subject.other | CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología | es |
dc.subject.other | CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica | es |
dc.title | Solubilization and characterization of a cell wall-bound trehalase from ascospores of the fission yeast Schizosaccharomyces pombe | es |
dc.type | info:eu-repo/semantics/article | es |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0944501307000419 | es |
dc.identifier.doi | https://doi.org/10.1016/j.micres.2006.12.005 | - |
Aparece en las colecciones: | Artículos: Genética y Microbiología |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Trehalase.pdf | 168,32 kB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons