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10.4049/jimmunol.180.11.7147
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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Pelegrin, Pablo | - |
dc.contributor.author | Barroso-Gutierrez, Consuelo | - |
dc.contributor.author | Surprenant, Annmarie | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular B e Inmunología | es |
dc.date.accessioned | 2024-01-25T09:28:48Z | - |
dc.date.available | 2024-01-25T09:28:48Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | The Journal of Immunology, volumen 180, nº 11, año 2008, páginas 7147-7157 | es |
dc.identifier.uri | http://hdl.handle.net/10201/137734 | - |
dc.description.abstract | The pro-inflammatory IL-1 cytokines, IL-1a, IL-1b and IL-18, are key mediators of the acute immune response to injury and infection. Mechanisms underlying their cellular release remain unclear. Activation of purinergic P2X7 receptors (P2X7R) by extracellular ATP is a key physiological inducer of rapid IL-1brelease from LPS-primed macrophage. We investigated patterns of ATP-mediated release of IL-1 cytokines from three macrophage types in attempts to provide direct evidence for or against distinct release mechanisms. We used peritoneal macrophage from P2X7R-/- mice and found that release of IL-1a, IL-18, as well as IL-1b, by ATP resulted exclusively from activation of P2X7R, that release of all these IL-1 cytokines involved pannexin-1 (panx1), and that there was both a panx1-dependent and independent component to IL-1b release. We compared IL-1 release patterns from LPS-primed peritoneal macrophage, RAW264.7 macrophage and J774A.1 macrophage. We found RAW264.7 macrophage readily release pro-IL- 1b independently of panx1 but do not release mature IL-1b because they do not express apoptotic speck-like protein with a caspase-activating recruiting domain (ASC) and so have no caspase-1 inflammasome activity. We delineated two distinct release pathways: the well-known caspase-1 cascade mediating release of processed IL-1b that was selectively blocked by inhibition of caspase-1 or panx1, and a calcium-independent, caspase-1/panx1-independent release of pro-IL-1b that was selectively blocked by glycine. None of these release responses were associated with cell damage or cytolytic effects. This provides the first direct demonstration of a distinct signaling mechanism responsible for ATP-induced release of pro-IL-1b. | es |
dc.format | application/pdf | es |
dc.format.extent | 39 | es |
dc.language | eng | es |
dc.publisher | The American Association of Immunologists, Inc. | es |
dc.relation | Wellcome Trust y AstraZeneca Charnwood, UK | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Ratones | es |
dc.subject | Macrófagos | es |
dc.subject | Citocinas | es |
dc.subject | LPS | es |
dc.subject | Inflamación | es |
dc.subject.other | CDU::6 - Ciencias aplicadas | es |
dc.title | P2X7 receptor differentially couples to distinct release pathways for IL-1b in mouse macrophage | es |
dc.type | info:eu-repo/semantics/article | es |
dc.relation.publisherversion | https://journals.aai.org/jimmunol/article/180/11/7147/84652/P2X7-Receptor-Differentially-Couples-to-Distinct | es |
dc.identifier.doi | 10.4049/jimmunol.180.11.7147 | - |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
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2008 JI.pdf | Versión aceptada del artículo | 3,86 MB | Adobe PDF | Visualizar/Abrir Solicitar una copia |
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