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10.1016/j.dci.2003.08.002
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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Pablo, Pelegrin | - |
dc.contributor.author | Elena, Chaves-Pozo | - |
dc.contributor.author | José, Meseguer | - |
dc.contributor.author | Victoriano, Mulero | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular B e Inmunología | es |
dc.date.accessioned | 2024-01-24T17:47:30Z | - |
dc.date.available | 2024-01-24T17:47:30Z | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | Developmental and Comparative Immunology, volumen 28, nº 3, año 2004, páginas 229-237 | es |
dc.identifier.issn | 0145-305X | - |
dc.identifier.uri | http://hdl.handle.net/10201/137694 | - |
dc.description | ©2004. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Developmental and Comparative Immunology (DCI). To access the final edited and published work see https://doi.org/10.1016/j.dci.2003.08.002 | es |
dc.description.abstract | Interleukin-1b (IL-1b) is a secretory cytokine lacking a signal peptide, and does not follow the classical endoplasmic reticulum to Golgi pathway of secretion. Its post-translational processing by IL-1b-converting enzyme (ICE) and subsequent release from activated macrophages requires ATP acting on P2X7 receptors. No information is available on the production and release of fish IL-1b, but the IL-1b gene sequences reported to date lack a conserved ICE recognition site. We show for the first time that lipopolysaccharide (LPS)/macrophage-activating factor (MAF)/bacterial DNA (VaDNA)-primed immune cells of fish accumulate intracellular IL-1b as a ~30 kDa polypeptide (proIL-1b). The combination of LPS and VaDNA was found to be synergistic, suggesting that each ligand is recognized by a different pattern recognition receptor (PRR). More importantly, addition of extracellular ATP does not promote IL-1b secretion by immune cells and fails to induce phosphatidylserine (PS) flip. In contrast, fish SAF-1 fibroblasts shed microvesicles containing a 22 kDa IL-1b form within 30 min of activation with ATP. Notably, the post-translational processing of IL-1b by SAF-1 cells is abrogated by a specific ICE inhibitor. | es |
dc.format | application/pdf | es |
dc.format.extent | 26 | es |
dc.language | eng | es |
dc.relation | Spanish Ministry of Science and Technology (grants PB98-0387 and BIO2001-2324-C02-02), Spanish Ministry of Education, Culture and Sport (fellowship to E. Chaves-Pozo) and Fundación Séneca, Coordination Centre for Research (grant PI-51/00782/FS/01 and fellowship to P. Pelegrín). | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Interleucina-1 | es |
dc.subject | ATP | es |
dc.subject | Citocinas | es |
dc.subject | Proteínas sin péptico señal | es |
dc.title | Production and mechanism of secretion of interleukin-1b from the marine fish gilthead seabream | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | 10.1016/j.dci.2003.08.002 | - |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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2004 Dev Comp Immunol IL-1.pdf | Versión aceptada del artículo | 3,65 MB | Adobe PDF | Visualizar/Abrir |
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