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10.1093/femsle/fnaa164
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Título: | An ideal spacing is required for the control of Class II CRP-dependent promoters by the status of CRP K100. |
Fecha de publicación: | 2020 |
Editorial: | Oxford Academic |
Cita bibliográfica: | FEMS Microbiology Letters, 367, 2020, fnaa164 |
ISSN: | 0378-1097 |
Palabras clave: | Escherichia coli Cyclic AMP receptor protein (CRP) Transcription activation Class II CRP-dependent promoters |
Resumen: | Transcription activation by the Escherichia coli CRP at Class II promoters is dependent on direct interactions between RNA polymerase and CRP, therefore the spatial proximity between both proteins plays a significant role in the ability of CRP to activate transcription. Using both in vivo and in vitro techniques, here we demonstrate that the CRP K100 positive charge, adjacent to AR2, is required for full promoter activity when CRP is optimally positioned. Accordingly, K100 mediated activation is very position-dependent and our data confirm that the largest impact of the K100 status on transcription activation occurs when the spacing between the CRP binding site and the A2 of the −10 element is 22 bp. From the results of this study and the progress in the understanding about open complex DNA scrunching, we propose that CRP-dependent promoters should now be numbered by the distance from the center of the DNA site for CRP and the most highly conserved base at position 2 of the −10 hexamer in bacterial promoters |
Autor/es principal/es: | Écija Conesa, Ana Gallego Jara, Julia Lozano Terol, Gema Browning, Douglas F. Busby, Steve J. W. Wolfe, Alan J. Canovas Diaz, Manuel De Diego Puente, Teresa |
URI: | http://hdl.handle.net/10201/136579 |
DOI: | 10.1093/femsle/fnaa164 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 9 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 International |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
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An ideal spacing is required for the control.pdf | 3,15 MB | Adobe PDF | Visualizar/Abrir |
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