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10.1093/femsle/fnaa164
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Écija Conesa, Ana | - |
dc.contributor.author | Gallego Jara, Julia | - |
dc.contributor.author | Lozano Terol, Gema | - |
dc.contributor.author | Browning, Douglas F. | - |
dc.contributor.author | Busby, Steve J. W. | - |
dc.contributor.author | Wolfe, Alan J. | - |
dc.contributor.author | Canovas Diaz, Manuel | - |
dc.contributor.author | De Diego Puente, Teresa | - |
dc.date.accessioned | 2023-12-12T23:11:55Z | - |
dc.date.available | 2023-12-12T23:11:55Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | FEMS Microbiology Letters, 367, 2020, fnaa164 | es_ES |
dc.identifier.issn | 0378-1097 | - |
dc.identifier.uri | http://hdl.handle.net/10201/136579 | - |
dc.description.abstract | Transcription activation by the Escherichia coli CRP at Class II promoters is dependent on direct interactions between RNA polymerase and CRP, therefore the spatial proximity between both proteins plays a significant role in the ability of CRP to activate transcription. Using both in vivo and in vitro techniques, here we demonstrate that the CRP K100 positive charge, adjacent to AR2, is required for full promoter activity when CRP is optimally positioned. Accordingly, K100 mediated activation is very position-dependent and our data confirm that the largest impact of the K100 status on transcription activation occurs when the spacing between the CRP binding site and the A2 of the −10 element is 22 bp. From the results of this study and the progress in the understanding about open complex DNA scrunching, we propose that CRP-dependent promoters should now be numbered by the distance from the center of the DNA site for CRP and the most highly conserved base at position 2 of the −10 hexamer in bacterial promoters | - |
dc.format | application/pdf | es_ES |
dc.format.extent | 9 | - |
dc.language | eng | es_ES |
dc.publisher | Oxford Academic | - |
dc.relation | Sin financiación externa a la Universidad | - |
dc.relation.isreferencedby | ED_IDENTRADA=1270 | - |
dc.rights | info:eu-repo/semantics/openAccess | - |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject | Escherichia coli | - |
dc.subject | Cyclic AMP receptor protein (CRP) | - |
dc.subject | Transcription activation | - |
dc.subject | Class II CRP-dependent promoters | - |
dc.title | An ideal spacing is required for the control of Class II CRP-dependent promoters by the status of CRP K100. | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1093/femsle/fnaa164 | - |
dc.archivorevisado | ©2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Submitted, Accepted, Published, version of a Published Work that appeared in final form in FEMS Microbiology Letters. To access the final edited and published work see doi:10.1093/femsle/fnaa164 | - |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
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An ideal spacing is required for the control.pdf | 3,15 MB | Adobe PDF | ![]() Visualizar/Abrir |
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