Por favor, use este identificador para citar o enlazar este ítem: 10.1007/s11259-023-10084-3

Título: What is the sensitivity and exactness of post-mortem diagnostic method for cardiopulmonary nematodes in wild carnivores? towards the gold standard
Fecha de publicación: 15-mar-2023
Editorial: Springer Nature
Cita bibliográfica: Veterinary Research Communications 47:1369-1378
Palabras clave: Artificial digestion
Post-mortem diagnostic
Red fox
Resumen: Cardiopulmonary nematodes cause health and fitness disorders in wild and domestic carnivores. The red fox (Vulpes vulpes) participates in the spread of these shared parasites at the domestic-wildlife interface. This study aimed to evaluate the sensitivity of post-mortem diagnostic method for detecting lungworms in carnivores, and its exactness to estimate the parasite intensity of each nematode species. Cardiorespiratory system of fifty-one foxes were examined through three consecutively methodological steps: first, the tracheobronchial tree, pulmonary arteries and their branches were opened (OT); next, lung parenchyma was immersed in water and squeezed (WS); finally, the parenchyma was artificially digested in a pepsin and chlorhydric acid solution (AD). Eucoleus aerophilus, Angiostrongylus vasorum, Crenosoma vulpis and Metathelazia capsulata were identified. The number of recovered nematodes in each step were 454 (OT), 285 (WS) and 141 (AD). The use of OT and WS helped to improve parasite intensity results and decreased false negative cases. Accordingly, when OT and WS were used together, the sensitivity in the detection of parasitized foxes was 96.1%, while the exactness of parasite intensity was 84%. When AD was performed, although sensitivity does not rise, results were more exact, increasing the total number of detected parasites by 16%. Moreover, AD improved the sensitivity in the detection of A. vasorum and M. capsulata, as well as quantifying more exactly the parasite intensity (92.5% and 92.3% of exactness without AD, respectively). Our study provides valuable information that should be taken into account when planning epidemiological studies based on cardiopulmonary nematode detection in carnivores.
Autor/es principal/es: Arcenillas Hernández, Irene
Ruiz de Ybáñez Carnero, María del Rocío
Martínez Carrasco-Pleite, Carlos
Facultad/Departamentos/Servicios: Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Sanidad Animal
Forma parte de: Proyecto: Papel epidemiológico del zorro como portador de agentes zoonósicos y de otros patógenos compartidos con carnívoros domésticos en ambientes antrópicos de clima mediterráneo semiárido
URI: http://hdl.handle.net/10201/136325
DOI: 10.1007/s11259-023-10084-3
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 10
Derechos: info:eu-repo/semantics/openAccess
Descripción: © 2023 The Author. This document is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/. This document is the published version of a Published Work that appeared in final form in Veterinary Research Communications. To access the final edited and published work see https://doi.org/10.1007/s11259-023-10084-3
Aparece en las colecciones:Artículos: Sanidad Animal

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