Por favor, use este identificador para citar o enlazar este ítem: DOI:10.2478/jvetres-2022-0010

Título: Comparison of commercial enzyme-linked immunosorbent assays for diagnosis of contagious agalactia caused by Mycoplasma agalactiae
Fecha de publicación: 10-mar-2022
Editorial: Sciendo
Walter de Gruyter GmbH
Cita bibliográfica: Journal of Veterinary Research, 66, Issue 1, 95-101, 2022
ISSN: 2450-8608
Materias relacionadas: CDU::6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca::636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos
Palabras clave: Contagious agalactia
Mycoplasma agalactiae
Enzyme-linked immunosorbent assays
Small ruminants
Goat
Resumen: Introduction: Contagious agalactia (CA) is a disease affecting small ruminants with worldwide distribution and caused by several mycoplasmas, especially M. agalactiae. The main option for systematic diagnosis under monitoring control programmes is the enzyme-linked immunosorbent assay (ELISA) test. Material and Methods: This study was designed to appraise the performance of two commercial indirect ELISA tests using M. agalactiae p48 protein and one using total protein, for antibody detection in small ruminants after natural infection with different M. agalactiae strains. We carried out the test evaluation using sera of confirmed M. agalactiae-positive goats with clinical signs. In addition, test agreement was assessed by kappa between the three commercial ELISA tests. Results: All three ELISA tests showed high validity scores (Youden’s J: 72.9–84%). The sensitivity values for the P48 protein-based tests were 76.9% and 84.6%, and was 79% for the total protein-based test. The specificity of all tests was 100%. In addition, between the total protein-based ELISA test and the other two ELISA tests based on the P48 protein, the agreement was substantial (kappa: 0.762–0.763) and the agreement between the latter two tests was almost perfect (kappa: 0.93). Conclusion: The validity parameters for all tests allowed their application for diagnostic purposes in lactating goats excreting M. agalactiae in milk and presenting clinical signs.
Autor/es principal/es: Sánchez López, Antonio
Contreras, Antonio
Sánchez-Corral, María L.
Martínez-Nista, Carmen
Collado, Soledad
Sáez, José L.
Minguez, Olga
de la Fe Rodríguez, Christian
Facultad/Departamentos/Servicios: Facultades, Departamentos, Servicios y Escuelas::Facultades de la UMU::Facultad de Veterinaria
Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Sanidad Animal
URI: http://hdl.handle.net/10201/135603
DOI: DOI:10.2478/jvetres-2022-0010
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 7
Derechos: info:eu-repo/semantics/openAccess
Descripción: © 2022 A. Sánchez et al. This document is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Journal of Veterinary Research. To access the final edited and published work see https://doi.org/10.2478/jvetres-2022-0010
Aparece en las colecciones:Artículos: Sanidad Animal

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