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DOI:10.2478/jvetres-2022-0010
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Sánchez López, Antonio | - |
dc.contributor.author | Contreras, Antonio | - |
dc.contributor.author | Sánchez-Corral, María L. | - |
dc.contributor.author | Martínez-Nista, Carmen | - |
dc.contributor.author | Collado, Soledad | - |
dc.contributor.author | Sáez, José L. | - |
dc.contributor.author | Minguez, Olga | - |
dc.contributor.author | de la Fe Rodríguez, Christian | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Facultades de la UMU::Facultad de Veterinaria | es |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Sanidad Animal | es |
dc.date.accessioned | 2023-11-13T07:07:35Z | - |
dc.date.available | 2023-11-13T07:07:35Z | - |
dc.date.issued | 2022-03-10 | - |
dc.identifier.citation | Journal of Veterinary Research, 66, Issue 1, 95-101, 2022 | es |
dc.identifier.issn | 2450-8608 | - |
dc.identifier.uri | http://hdl.handle.net/10201/135603 | - |
dc.description | © 2022 A. Sánchez et al. This document is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Journal of Veterinary Research. To access the final edited and published work see https://doi.org/10.2478/jvetres-2022-0010 | - |
dc.description.abstract | Introduction: Contagious agalactia (CA) is a disease affecting small ruminants with worldwide distribution and caused by several mycoplasmas, especially M. agalactiae. The main option for systematic diagnosis under monitoring control programmes is the enzyme-linked immunosorbent assay (ELISA) test. Material and Methods: This study was designed to appraise the performance of two commercial indirect ELISA tests using M. agalactiae p48 protein and one using total protein, for antibody detection in small ruminants after natural infection with different M. agalactiae strains. We carried out the test evaluation using sera of confirmed M. agalactiae-positive goats with clinical signs. In addition, test agreement was assessed by kappa between the three commercial ELISA tests. Results: All three ELISA tests showed high validity scores (Youden’s J: 72.9–84%). The sensitivity values for the P48 protein-based tests were 76.9% and 84.6%, and was 79% for the total protein-based test. The specificity of all tests was 100%. In addition, between the total protein-based ELISA test and the other two ELISA tests based on the P48 protein, the agreement was substantial (kappa: 0.762–0.763) and the agreement between the latter two tests was almost perfect (kappa: 0.93). Conclusion: The validity parameters for all tests allowed their application for diagnostic purposes in lactating goats excreting M. agalactiae in milk and presenting clinical signs. | es |
dc.format | application/pdf | es |
dc.format.extent | 7 | es |
dc.language | eng | es |
dc.publisher | Sciendo | es |
dc.publisher | Walter de Gruyter GmbH | - |
dc.relation | This research was funded by the Spanish Ministry of Agriculture with the technical collaboration of the Consejería de Agricultura, Ganadería y Desarrollo Rural de la Junta de Castilla y León (The Agriculture, Livestock, Fisheries and Rural Development Council of the Castilla y Leon Administrative Authority). | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Contagious agalactia | es |
dc.subject | Mycoplasma agalactiae | es |
dc.subject | Enzyme-linked immunosorbent assays | es |
dc.subject | Small ruminants | es |
dc.subject | Goat | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca::636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos | es |
dc.title | Comparison of commercial enzyme-linked immunosorbent assays for diagnosis of contagious agalactia caused by Mycoplasma agalactiae | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | DOI:10.2478/jvetres-2022-0010 | - |
Aparece en las colecciones: | Artículos: Sanidad Animal |
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Fichero | Descripción | Tamaño | Formato | |
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Sánchez 2022 ELISAs Ma J Vet Res.pdf | Artículo | 309,35 kB | Adobe PDF | ![]() Visualizar/Abrir |
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