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10.1021/acs.jafc.9b01617
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Título: | Microwave assisted cloud point extraction for the determination of vitamin K homologues in vegetables by liquid chromatography with tandem mass spectrometry |
Fecha de publicación: | 16-may-2019 |
Editorial: | ACS |
Cita bibliográfica: | Journal of Agricultural and Food Chemistry, 2019, 67(23), 6658-6664 |
ISSN: | 0021-8561 |
Materias relacionadas: | CDU::5 - Ciencias puras y naturales::54 - Química::543 - Química analítica |
Palabras clave: | cloud point extraction K vitamins liquid chromatography-tandem mass spectrometry menadione menaquinone phylloquinone vegetables |
Resumen: | Liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) with a triple quadrupole (QqQ) is proposed for determining the vitamin K homologues, phylloquinone (PK), menaquinone-4 (MK) and menadione (MD), in vegetables. The analytes were isolated from the samples (1−1.5 g) by ultrasound assisted extraction using acetonitrile (2 mL), and the liquids were submitted to microwave assisted cloud point extraction with Triton X-45. The enrichment factors were between 20 and 50, depending on the vitamin homologue in question. The analytes were determined by LC-ESI-QqQ-MS/MS in the multiple reaction monitoring (MRM) mode, providing unequivocal identification and quantification, with limits of detection of 0.8, 1.0, and 16 ng/g for MK, PK, and MD, respectively. Recovery assays for samples spiked at two concentration levels, between 40 and 600 ng/g depending on the compound, provided recoveries in the 90−114% range. Only PK was detected in the samples analyzed, at concentrations in the 90−2350 ng/g range. |
Autor/es principal/es: | Campillo, N. Marín, J. Viñas López-Pelegrín, Pilar Garrido, I. Fenoll, J. Hernández Córdoba, Manuel |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Química Analítica |
URI: | http://hdl.handle.net/10201/113646 |
DOI: | 10.1021/acs.jafc.9b01617 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 32 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
Aparece en las colecciones: | Artículos: Química Analítica |
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