Por favor, use este identificador para citar o enlazar este ítem: 10.1093/femsle/fnaa164.

Título: An ideal spacing is required for the control of Class II CRP-dependent promoters by the status of CRP K100
Fecha de publicación: 11-nov-2020
Cita bibliográfica: FEMS Microbiol Lett. (2020) 367(20):fnaa164
ISSN: 0378-1097
Materias relacionadas: CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica
Palabras clave: Escherichia coli
cyclic AMP receptor protein (CRP)
transcription activation
class II CRP-dependent promoters
Resumen: Transcription activation by the Escherichia coli CRP at Class II promoters is dependent on direct interactions between RNA polymerase and CRP, therefore the spatial proximity between both proteins plays a significant role in the ability of CRP to activate transcription. Using both in vivo and in vitro techniques, here we demonstrate that the CRP K100 positive charge, adjacent to AR2, is required for full promoter activity when CRP is optimally positioned. Accordingly, K100 mediated activation is very position-dependent and our data confirm that the largest impact of the K100 status on transcription activation occurs when the spacing between the CRP binding site and the A2 of the -10 element is 22 bp. From the results of this study and the progress in the understanding about open complex DNA scrunching, we propose that CRP-dependent promoters should now be numbered by the distance from the centre of the DNA site for CRP and the most highly conserved base at position 2 of the -10 hexamer in bacterial promoters.
Autor/es principal/es: Ecija Conesa, Ana
Gallego Jara, Julia
Lozano-Terol, Gema
Browning, Douglas F.
Busby, Steve J.W.
Wolfe, Alan J.
Cánovas Díaz, Manuel
de Diego Puente, Teresa Teresa
Facultad/Departamentos/Servicios: Department of Biochemistry and Molecular Biology (B)and Immunology , Faculty of Chemistry, University of Murcia
Versión del editor: https://academic.oup.com/femsle/article-abstract/367/20/fnaa164/5936555?redirectedFrom=fulltext
URI: http://hdl.handle.net/10201/113632
DOI: 10.1093/femsle/fnaa164.
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 26
Derechos: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Aparece en las colecciones:Artículos: Bioquímica y Biología Molecular "B" e Inmunología

Ficheros en este ítem:
Fichero Descripción TamañoFormato 
FEMSLE-20-06-0296.Proof_hi.pdf2,2 MBAdobe PDFVista previa
Visualizar/Abrir


Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons Creative Commons