Por favor, use este identificador para citar o enlazar este ítem:
10.3389/fcell.2020.588851
![](/digitum/image/email_logo.png)
![](/digitum/image/logo-facebook.png)
Título: | Netrin-1/DCC signaling differentially regulates the migration of Pax7, Nkx6.1, Irx2, Otp and Otx2 cell populations in the developing interpeduncular nucleus |
Fecha de publicación: | 20-oct-2020 |
Fecha de defensa / creación: | 2020 |
Editorial: | Frontiers Media |
Cita bibliográfica: | Frontiers in Cell and Developmental Biology, 2020 8:588851 Front. Cell Dev. Biol.. |
ISSN: | 2296-634X |
Materias relacionadas: | CDU::5 - Ciencias puras y naturales |
Palabras clave: | IPN Interpeduncular nucleus Prodromal Interpeduncular rostral Interpeduncular caudal Isthmus Rrhombomere 1 Medial habenula Rhombomere 2 Rhombomere 1 rostral Rhombomere 1 caudal |
Resumen: | The interpeduncular nucleus (IPN) is a hindbrain structure formed by three main subdivisions, the prodromal (Pro) domain located at the isthmus (Ist), and the rostral and caudal interpeduncular domains (IPR, IPC) within rhombomere 1 (r1). Various cell populations can be detected in the IPN through the expression of the Nkx6.1, Otp, Otx2, Pax7, and/or Irx2 transcription factors. These cell populations follow independent dorsoventral tangential and radial migratory routes targeting the ventral paramedian region of Ist and r1. Here we set out to examine the influence of the Netrin-1/DCC pathway on these migrations, since it is known to regulate other processes of neuronal migration in the brain. To this end, we analyzed IPN development in late gestational wild-type and DCC-/- mice, using mainly in situ hybridization (ISH) to identify the cells expressing each of the aforementioned genes. We found that the migration of Nkx6.1 + and Irx2 + cells into the Pro domain was strongly disrupted by the loss of DCC, as occurred with the migration of Pax7 +, Irx2 +, and Otp + cells that would normally form the IPR. In addition, there was mild impairment of the migration of the Pax7 + and Otx2 + cells that form the IPC. These results demonstrate that the Netrin-1/DCC signaling pathway is involved in the migration of most of the IPN populations, mainly affecting those of the Pro and IPR domains of this nucleus. There are psychiatric disorders that involve the medial habenula (mHb)-IPN system, so that this experimental model could provide a basis to study their neurodevelopmental etiology. |
Autor/es principal/es: | García-Guillén, Isabel M. Alonso, Antonia Morales-Delgado, Nicanor Andrés, Belén Puelles López, Luis López-Bendito, Guillermina Marín, Faustino Aroca Tejedor, Pilar |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Anatomía Humana y Psicobiología |
Versión del editor: | https://www.frontiersin.org/articles/10.3389/fcell.2020.588851/full |
URI: | http://hdl.handle.net/10201/105581 |
DOI: | 10.3389/fcell.2020.588851 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 11 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional Atribución 4.0 Internacional |
Descripción: | © 2020. The authors. This document is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/ This document is the Published version of a Published Work that appeared in final form in Frontiers in Cell and Developmental Biology. To access the final edited and published work see https://doi.org/10.3389/fcell.2020.588851 |
Aparece en las colecciones: | Artículos: Anatomía Humana y Psicobiología |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
García-Guillen et al-2020.pdf | 5,47 MB | Adobe PDF | ![]() Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons