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Título: | Endoglin co-expression with eNOS, SMAD2 and phosphorylated SMAD23 in normocholesterolemic and hypercholesterolemic mice: an immunohistochemical study |
Fecha de publicación: | 2009 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | 615 - Farmacología. Terapéutica. Toxicología. Radiología |
Palabras clave: | Immunohistochemistry Mice |
Resumen: | Endoglin, a homodimeric transmembrane glycoprotein, is a part of the transforming growth factorß (TGF-ß) receptor cascade. It has been demonstrated that endoglin can affect TGF-ß signaling and eNOS expression by affecting SMAD proteins in vitro. We planned to go one step forward and evaluate whether endoglin is co-expressed with SMAD2, phosphorylated SMAD2/3 protein and eNOS in endothelium of normocholesterolemic C57BL/6J mice, and in advanced atherosclerotic lesions in hypercholesterolemic apoE/LDLr-deficient mice by means of fluorescence immunohistochemistry. Female C57BL/6J mice were fed with a chow diet (standard laboratory diet) for 12 weeks after weaning (at the age of 4 weeks). Two-month-old female apoE/LDLrdeficient mice were fed the western type diet (atherogenic diet) containing 21% fat (11% saturated fat) and 0.15% cholesterol for 2 months. Immunohistochemical analysis of endoglin, SMAD2, phosphorylated SMAD2/3 and eNOS expression was performed in mice aortic sinus. Immunohistochemical analysis showed the expression of endoglin in intact endothelium in both C57BL/6J and apoE/LDLr-deficient mice and in endothelium covering the atherosclerotic lesion in apoE/LDLr-deficient mice. Fluorescence immunohistochemistry revealed co-expression of endoglin with SMAD2, phosphorylated SMAD2/3 and eNOS in intact aortic endothelium in C57BL/6J mice. Moreover, strong co-localization of endoglin, SMAD2, phosphorylated SMAD2/3 and eNOS was also detected in endothelium covering atherosclerotic lesions in apoE/LDLr-deficient mice. In conclusion, we suggest that endoglin, SMAD2, phosphorylated SMAD2/3 and eNOS may be important in vessel endothelium homeostasis underlying their role in atherogenesis. |
Autor/es principal/es: | Nachtigal, Peter Vecerova, lenka Pospisilova, Nada Micuda, Stanislav Brcakova, Eva Navarro Hernandez, Elena Pospechova, Katerina Semecky, Vladimir |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/37552 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 8 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.24,nº12 (2009) |
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