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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Nachtigal, Peter | es |
dc.contributor.author | Vecerova, lenka | es |
dc.contributor.author | Pospisilova, Nada | - |
dc.contributor.author | Micuda, Stanislav | - |
dc.contributor.author | Brcakova, Eva | - |
dc.contributor.author | Navarro Hernandez, Elena | - |
dc.contributor.author | Pospechova, Katerina | - |
dc.contributor.author | Semecky, Vladimir | - |
dc.date.accessioned | 2014-01-09T10:39:02Z | - |
dc.date.available | 2014-01-09T10:39:02Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0213-3911 | es |
dc.identifier.uri | http://hdl.handle.net/10201/37552 | - |
dc.description.abstract | Endoglin, a homodimeric transmembrane glycoprotein, is a part of the transforming growth factorß (TGF-ß) receptor cascade. It has been demonstrated that endoglin can affect TGF-ß signaling and eNOS expression by affecting SMAD proteins in vitro. We planned to go one step forward and evaluate whether endoglin is co-expressed with SMAD2, phosphorylated SMAD2/3 protein and eNOS in endothelium of normocholesterolemic C57BL/6J mice, and in advanced atherosclerotic lesions in hypercholesterolemic apoE/LDLr-deficient mice by means of fluorescence immunohistochemistry. Female C57BL/6J mice were fed with a chow diet (standard laboratory diet) for 12 weeks after weaning (at the age of 4 weeks). Two-month-old female apoE/LDLrdeficient mice were fed the western type diet (atherogenic diet) containing 21% fat (11% saturated fat) and 0.15% cholesterol for 2 months. Immunohistochemical analysis of endoglin, SMAD2, phosphorylated SMAD2/3 and eNOS expression was performed in mice aortic sinus. Immunohistochemical analysis showed the expression of endoglin in intact endothelium in both C57BL/6J and apoE/LDLr-deficient mice and in endothelium covering the atherosclerotic lesion in apoE/LDLr-deficient mice. Fluorescence immunohistochemistry revealed co-expression of endoglin with SMAD2, phosphorylated SMAD2/3 and eNOS in intact aortic endothelium in C57BL/6J mice. Moreover, strong co-localization of endoglin, SMAD2, phosphorylated SMAD2/3 and eNOS was also detected in endothelium covering atherosclerotic lesions in apoE/LDLr-deficient mice. In conclusion, we suggest that endoglin, SMAD2, phosphorylated SMAD2/3 and eNOS may be important in vessel endothelium homeostasis underlying their role in atherogenesis. | es |
dc.format | application/pdf | es |
dc.format.extent | 8 | es |
dc.language | eng | es |
dc.publisher | Murcia : F. Hernández | es |
dc.relation.ispartof | Histology and histopathology | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Immunohistochemistry | es |
dc.subject | Mice | es |
dc.subject.other | 615 - Farmacología. Terapéutica. Toxicología. Radiología | es |
dc.title | Endoglin co-expression with eNOS, SMAD2 and phosphorylated SMAD23 in normocholesterolemic and hypercholesterolemic mice: an immunohistochemical study | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.24,nº12 (2009) |
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Endoglin coexpression with eNOS SMAD2 and phosphorylated SMAD23 in.pdf | 7,31 MB | Adobe PDF | Visualizar/Abrir |
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