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https://doi.org/10.3389/fvets.2023.1102049
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Título: | Extracellular vesicles would be involved in the release and delivery of seminal TGF-β isoforms in pigs |
Fecha de publicación: | 10-feb-2023 |
Editorial: | Frontiers Media |
Cita bibliográfica: | Front. Vet. Sci. 10:1102049 |
ISSN: | Electronic: 2297-1769 |
Palabras clave: | Extracellular vesicles Porcine Semen Seminal plasma Sperm TGFβ isoforms |
Resumen: | Introduction: pig seminal plasma (SP) is rich in active forms of all three isoforms (1-3) of transforming growth factor β (TGF-β), a chemokine modulatory of the immune environment in the female genital tract once semen is delivered during mating or artificial insemination (AI). The present study aimed to examine how TGF-βs are secreted by the epithelium of the male reproductive tract and how they are transported in semen, emphasizing the interplay with seminal extracellular vesicles (sEVs). Methods: Source of TGF-βs was examined by immunohistochemistry in testis, epididymis, and accessory sex glands, by immunocytochemistry in ejaculated spermatozoa, and by Luminex xMAP® technology in SP and sEVs retrieved from healthy, fertile male pigs used as breeders in AI programs. Results: All three TGF-β isoforms were expressed in all reproductive tissues explored and would be released into ductal lumen either in soluble form or associated with sEVs. Ejaculated spermatozoa expressed all three TGF-β isoforms, both inside and outside, probably the outer one associated with membrane-bound sEVs. The results confirmed that pig SP contains all three TGF-β isoforms and demonstrated that a substantial portion of them is associated with sEVs. Discussion: Seminal EVs would be involved in the cellular secretion of the active forms of seminal TGF-β isoforms and in their safe transport from the male to the female reproductive tract. |
Autor/es principal/es: | Padilla, Lorena Barranco, Isabel Martínez Hernández, Jesús Parra, Ana Parrilla, Inmaculada Pastor, Luis Miguel Rodríguez Martínez, Heriberto Lucas, Xiomara Roca, Jordi |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Medicina y Cirugía Animal |
Versión del editor: | https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1102049/full |
URI: | http://hdl.handle.net/10201/143606 |
DOI: | https://doi.org/10.3389/fvets.2023.1102049 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 13 |
Derechos: | info:eu-repo/semantics/openAccess Atribución 4.0 Internacional |
Descripción: | © 2023 Padilla, Barranco, Martínez-Hernández, Parra, Parrilla, Pastor, Rodriguez-Martinez, Lucas and Roca. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in Frontiers in Veterinary Science. To access the final edited and published work see https://doi.org/10.3389/fvets.2023.1102049 |
Aparece en las colecciones: | Artículos: Medicina y Cirugía Animal |
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