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https://doi.org/10.3389/fvets.2023.1102049
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Padilla, Lorena | - |
dc.contributor.author | Barranco, Isabel | - |
dc.contributor.author | Martínez Hernández, Jesús | - |
dc.contributor.author | Parra, Ana | - |
dc.contributor.author | Parrilla, Inmaculada | - |
dc.contributor.author | Pastor, Luis Miguel | - |
dc.contributor.author | Rodríguez Martínez, Heriberto | - |
dc.contributor.author | Lucas, Xiomara | - |
dc.contributor.author | Roca, Jordi | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Medicina y Cirugía Animal | es |
dc.date.accessioned | 2024-09-05T09:58:09Z | - |
dc.date.available | 2024-09-05T09:58:09Z | - |
dc.date.issued | 2023-02-10 | - |
dc.identifier.citation | Front. Vet. Sci. 10:1102049 | es |
dc.identifier.issn | Electronic: 2297-1769 | - |
dc.identifier.uri | http://hdl.handle.net/10201/143606 | - |
dc.description | © 2023 Padilla, Barranco, Martínez-Hernández, Parra, Parrilla, Pastor, Rodriguez-Martinez, Lucas and Roca. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in Frontiers in Veterinary Science. To access the final edited and published work see https://doi.org/10.3389/fvets.2023.1102049 | - |
dc.description.abstract | Introduction: pig seminal plasma (SP) is rich in active forms of all three isoforms (1-3) of transforming growth factor β (TGF-β), a chemokine modulatory of the immune environment in the female genital tract once semen is delivered during mating or artificial insemination (AI). The present study aimed to examine how TGF-βs are secreted by the epithelium of the male reproductive tract and how they are transported in semen, emphasizing the interplay with seminal extracellular vesicles (sEVs). Methods: Source of TGF-βs was examined by immunohistochemistry in testis, epididymis, and accessory sex glands, by immunocytochemistry in ejaculated spermatozoa, and by Luminex xMAP® technology in SP and sEVs retrieved from healthy, fertile male pigs used as breeders in AI programs. Results: All three TGF-β isoforms were expressed in all reproductive tissues explored and would be released into ductal lumen either in soluble form or associated with sEVs. Ejaculated spermatozoa expressed all three TGF-β isoforms, both inside and outside, probably the outer one associated with membrane-bound sEVs. The results confirmed that pig SP contains all three TGF-β isoforms and demonstrated that a substantial portion of them is associated with sEVs. Discussion: Seminal EVs would be involved in the cellular secretion of the active forms of seminal TGF-β isoforms and in their safe transport from the male to the female reproductive tract. | es |
dc.format | application/pdf | es |
dc.format.extent | 13 | es |
dc.language | eng | es |
dc.publisher | Frontiers Media | - |
dc.relation | This study was funded by the Spanish Ministry of Science and Innovation (grant PID2020-113493RB-I00 funded by MCIN/AEI/10.13039/501100011033), Madrid, Spain; the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 891382; and FORMAS, Stockholm (grant 2019-00288). | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Extracellular vesicles | es |
dc.subject | Porcine | - |
dc.subject | Semen | - |
dc.subject | Seminal plasma | - |
dc.subject | Sperm | - |
dc.subject | TGFβ isoforms | - |
dc.title | Extracellular vesicles would be involved in the release and delivery of seminal TGF-β isoforms in pigs | es |
dc.type | info:eu-repo/semantics/article | es |
dc.relation.publisherversion | https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1102049/full | es |
dc.identifier.doi | https://doi.org/10.3389/fvets.2023.1102049 | - |
Aparece en las colecciones: | Artículos: Medicina y Cirugía Animal |
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fvets-10-1102049.pdf | 3,37 MB | Adobe PDF | Visualizar/Abrir |
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