Browsing by Subject "lipase"

Now showing 1 - 3 of 3
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    Biocatalytic Synthesis of Polyglycerol Polyricinoleate: A Comparison of Different Commercial Lipases
    (Assoc. of Chemists and Chemical Engineers of Croatia, 2013-10) Ortega Requena, Salvadora; Gómez, José Luis; Bastida Rodríguez, Josefa; Máximo Martín, María Fuensanta; Montiel Morte, María Claudia; Gómez, María; Ingeniería Química
    In this work, the studies were carried out to select the most suitable lipase as catalyst for the esterification of polyglycerol with polyricinoleic acid to yield polyglicerol polyricinoleate (PGPR), a valued-added, biobased food emulsifier are described. The enzymes assayed were lipases from Rhizopus arrhizus, Rhizopus oryzae and Mucor javanicus, previously selected because of their suitable activity and moderate cost. First, reaction was catalyzed by free lipases in a batch reactor and the influence of different operating conditions (initial water content, amount of enzyme and temperature) on the progress of the reaction was studied. Next, the three lipases were immobilized by physical adsorption on the anion exchange resin, Lewatit MonoPlus MP 64, providing derivatives with a high activity and stability. Recovery of the immobilized derivative from the reaction medium was conducted with very good yields (up 99%) and no loss of activity of the derivative with successive uses was proved. Finally, a high performance reactor, which operates at low pressure and with a dry atmosphere, was used to synthesise PGPR using the immobilized enzymes. Both Rhizopus arrhizus and Rhizopus oryzae lipases allowed the production of a PGPR which fulfils the “specific purity criteria on food additives other than colours and sweeteners” established by the Commission of the European Communities, with an acid value of 4.91 and 5.31 mg KOH/g respectively.
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    Kinetic modelling and kinetic parameters calculation in the lipase-catalysed 1 synthesis of geranyl acetate.
    (Elsevier, 2018-08-22) Murcia Almagro, María Dolores; Gómez Gómez, María; Gómez E.; J.L. Gómez; A.M. Hidalgo; A. Sánchez; P. Vergara; Ingeniería Química
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    PRODUCTION OF RICINOLEIC ACID ESTOLIDE WITH FREE AND IMMOBILIZED LIPASE FROM Candida rugosa.
    (2007-10-23) Bódalo, A.; Bastida Rodríguez, Josefa; Máximo Martín, María Fuensanta; Montiel Morte, María Claudia; Gómez, M.; Murcia Almagro, María Dolores; Ingeniería Química
    Ricinoleic acid estolide was produced by using free and immobilized Candida rugosa lipase at moderate temperature in a bioreactor. This work describes the immobilization of Candida rugosa lipase on ten different supports by covalent binding and physical adsorption, and how of the most suitable immobilized derivative was selected. The comparison was mainly based on the enzyme content and on the activity results. An anion exchange resin was judged to be the most appropriate support and the corresponding immobilization process was investigated and optimized. Although repeated batch reactions using the same derivative are not entirely advisable, the reaction proceeds at a noticeably slower rate and the degree of condensation reached is lower when the same amount of protein as in the derivative is added to the bioreactor in native form.