Browsing by Subject "Tissues"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Publication
    Open Access
    lmmunocytochemical distribution of cytochrome P4501A CYPlA in developing gilthead seabream, Sparus aurata
    (Murcia : F. Hernández, 1999) Sarasquete, C.; Muñoz-Cueto, J.A.; Ortiz Delgado, J.B.; Rodriguez-Gomez, F.J.; Dinis, M.T.; Segner, H.
    CYPlA is a major inducible enzyme in the metabolism of xenobiotic substrates. In this paper we investigate by means of immunohistochemistry, the tissue distribution of constitutive cytochrome P4501A (CYPlA) during the period of endogenous nutrition (from hatching until day 4) in developing gilthead seabream, Sparus aurata larvae. For this purpose, a polyclonal antiserum (BN- 1, Biosense Laboratories) directed against conserved piscine CYPlA sequences was used on paraffin-embedded sections from seabream larvae. From hatching onward, CYPlA immunoreactivity was observed in the following tissues and cells: syncytial, oil-globule envelopes and matrix of the yolk-sac, kidney (epithelia of renal tubules), cardiac muscle cells, skin epidermal cells, troncal musculature, enterocytes of different intestinal regions, goblet cells of the bucco-pharyngeal region, gill epithelia1 cells and the endothelia of the vascular system of various tissues (especially from liver and brain). Moreover, eye (retina), olfactory epithelium and some positive nerve fibers located in the proximity of the olfactory bulbs and running ventrally toward the posterior brain were strongly CYPlA immunoreactive. In general, the intensity of immunostaining increased with larval development.
  • Thumbnail Image
    Publication
    Embargo
    Metals and metalloids in whole blood and tissues of Olive Ridley turtles (Lepidochelys olivacea) from La Escobilla Beach (Oaxaca, Mexico)
    (Elsevier, 2014-10-06) Cortés-Gómez, Adriana A.; Fuentes-Mascorro, Gisela; Romero García, Diego; Ciencias Sociosanitarias
    Concentrations of eight metals and metalloids (Pb, Cd, Cu, Zn, Mn, Se, Ni and As) were evaluated from 41 nesting females (blood) and 13 dead (tissues) Olive Ridley turtles (Lepidochelys olivacea), a species classified as vulnerable and also listed in Appendix I of the Convention of International Trade in Endangered Species (CITES). The mean blood, liver and kidney lead concentration were 0.02 ± 0.01, 0.11 ± 0.08 and 0.06 ± 0.03 μg g−1 ww respectively, values lower than other turtle species and locations, which it could be due to the gradual disuse of leaded gasoline in Mexico and Central America since the 1990s. Mean concentration of cadmium was 0.17 ± 0.08 (blood), 82.88 ± 36.65 (liver) and 150.88 ± 110.99 μg g−1 (kidney). To our knowledge, the mean renal cadmium levels found is the highest ever reported worldwide for any sea turtle species, while other six elements showed a concentration similar to other studies in sea turtles.
  • Thumbnail Image
    Publication
    Open Access
    Tissue Distribution of Mercury and Its Relationship with Selenium in Atlantic Bluefin Tuna (Thunnus thynnus L.)
    (MDPI, ) Belmonte, Antonio; Muñoz, Pilar; Santos Echeandía, Juan; Romero García, Diego; Sanidad Animal; Ciencias Sociosanitarias