Browsing by Subject "Sulforaphene"

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    Bioavalability and new biomarkers of cruciferous sprouts consumption
    (Elsevier, 2017-07-21) Baenas, Nieves; Suárez-Martínez, Clara; García-Viguera, Cristina; Moreno, Diego A.; Tecnología de Alimentos, Nutrición y Bromatología
    The evaluation of the bioavailability of cruciferous compounds is one of several challenges in the design of clinical trials. A 7-days-cross-over study with fourteen women was undertaken to compare the bioavailability of glucosinolates from broccoli and radish sprouts. The urinary excretion of isothiocyanates, indoles and metabolites was analysed by UHPLC-QqQ-MS/MS. Even though the bioavailability of broccoli compounds has been studied, as far as we are aware, there are not any biomarkers established for radish sprouts intake. For the first time, sulforaphene, sulforaphane-N-acetyl-L-cysteine (SFN-NAC) and 3,3’diindolylmethane (DIM), were studied as biomarkers of dietary exposure to radish. The SFN-NAC and DIM were already considered biomarkers of broccoli consumption. Higher excretion of conjugated isothiocyanates and homogeneous excretion of indoles were found during the first 12 h after ingestion. Metabolites were excreted homogeneously during the study, suggesting no accumulation. These results provide valuable information to better understand the bioavailability of cruciferous bioactives.
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    Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster.
    (MDPI, 2016-02-18) Baenas, Nieves; Piegholdt, Stefanie; Schloesser, Anke; Moreno, Diego A.; García-Viguera, Cristina; Rimbach, Gerald; Wagner, Anika E.; Tecnología de Alimentos, Nutrición y Bromatología
    We used Drosophila melanogaster as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (Raphanus sativus cv. Rambo), a Brassicaceae species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/L) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that Drosophila melanogaster takes up and metabolizes isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of spargel expression, the Drosophila homolog of the mammalian PPARγ-coactivator 1 α, as well as the inhibition of α-amylase and α-glucosidase in vitro. Overall, we show that the consumption of radish sprouts affects energy metabolism in Drosophila melanogaster which is reflected by lower glucose levels and an increased expression of spargel, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellitus