Browsing by Subject "Skin"
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- PublicationOpen AccessCritical role of IkB kinase alpha in embryonic skin development and skin carcinogenesis(Murcia : F. Hernández, 2009) Zhu, Feng; Park, Eunmi; Liu, Bigang; Xia, Xiaojun; Fischer, Susan M.; Hu, YinlingIκB kinase alpha (IKKα), IKKß, and IKKγ/NEMO form the IKK complex, which is essential for NF-κB activation. However, genetic studies have shown that the role of IKKα is distinct from that of IKKß or IKKγ in the development of the mouse embryonic skin. Loss of IKKα has been shown to cause epidermal hyperplasia, prevent keratinocyte terminal differentiation, and impair the formation of the skin, resulting in the deaths of IKKα-deficient (Ikkα -/-) mice soon after birth. Recent experimental data from several laboratories have revealed that IKKα functions as a tumor suppressor in human squamous cell carcinomas (SCCs) of skin, lungs, and head and neck. Chemical carcinogenesis studies using mice have shown that reduction in IKKα expression increases the number and size of Ras-initiated skin tumors and promotes their progression, indicating that reduced IKKα expression provides a selective growth advantage that cooperates with Ras activity to promote skin carcinogenesis. In this review, we will summarize these findings from our and other studies on the role that IKKα plays in development of the mouse embryonic skin and skin carcinogenesis
- PublicationOpen AccessDataset used for Report of "Proteomic changes induced by hepcidin 1 injection in brain and skin of gilthead seabream (Sparus aurata)"(2025) Claudia Marín-Parra; Laura García-Navarro; Serna Duque, John Alberto; M. Ángeles Esteban; Biología Celular e Histología; M. Ángeles EstebanThe present study investigated the effects of hepcidin 1 (Hamp1) on the proteome of brain and skin of gilthead seabream (Sparus aurata). Hepcidin 1, a host defense peptide with antimicrobial and immunomodulatory functions, was inyected intraperitoneally at a dose of 25 μM. Proteomic analyses of brain and skin samples were performed using HPLC-MS/MS at 4 and 10 days post-injection. Data processing and analysis revealed significant alterations in protein expression profiles in both tissues. In the brain, hepcidin 1 injection upregulated proteins involved in DNA repair, myelin sheath structure, and extracellular matrix remodeling at 4 days, while at 10 days, changes were observed in proteins involved in endoplasmic reticulum calcium channels, Golgi apparatus glycosylation, and cytoskeletal dynamics. Skin samples exhibited increased expression of proteins related to endoplasmic reticulum stress response, cell adhesion, and vesicular trafficking at 4 days. At 10 days, skin proteome alterations indicated a metabolic shift towards enhanced glycolysis, oxidative stress adaptation, and cytoskeletal remodeling. These findings suggest that hepcidin 1 induces tissue-specific and time-dependent proteomic changes in gilthead seabream, potentially modulating immune responses, energy metabolism, and cellular adaptation processes. Further research is needed to elucidate the functional implications of these proteomic alterations and their potential applications in aquaculture health management.
- PublicationOpen AccessDifferential expression of tissue kallikrein in the skin of systemic sclerosis(Murcia : F. Hernández, 2005) Milia, A.F.; Del Rosso, A.; Pacini, A.; Manetti, M.; Marrelli, A.; Nosi, Daniele; Giacomelli, R.; Matucci-Cerinic, M.; Ibba-Manneschi, L.Systemic sclerosis (SSc) is characterised by ischemic damage, impaired angiogenesis and skin fibrosis. Tissue kallikrein (t-kallikrein) is involved through kinins in inflammation, vasorelaxation and angiogenesis. T-kallikrein is synthetised by endothelial, smooth muscle, and inflammatory cells and, in skin, also by dark cells of the sweat glands, where it is involved in sweat formation. Our aim was to analyse, by immunohistochemistry and RT-PCR, the expression of t-kallikrein in the skin of patients with different SSc subsets, limited (lSSc) and diffuse (dSSc), and phases, early and advanced. Skin biopsies were taken from 18 SSc patients and 10 controls. Immunohistochemistry was performed on paraffin sections with an antibody against human urinary t-kallikrein. For RT-PCR, cDNA from skin biopsies was amplified using primers specific for human t-kallikrein. In the control skin, dark cells of the secretory units of sweat glands showed immunopositivity for tkallikrein as well as blood vessels. In the lSSc skin, immunoreactivity was observed only in some glands, with weak staining in the advanced phase. In early lSSc skin, immunoreactivity was observed in microvessel walls and in the inflammatory infiltrate. In dSSc skin, dark cells of the glandular fundus units, and the few remaining vessels showed scarcity (early phase) or lack (advanced phase) of immunoreactivity for t-kallikrein. RT-PCR confirmed a decrease of t-kallikrein mRNA levels from early to advanced phase in SSc subsets, reaching its lowest level in advanced dSSc. In conclusion, immunohistochemical and biomolecular results indicate that t-kallikrein is decreased in the skin of SSc patients and decreases progressively from the early to advanced phase of lSSc and dSSc. The decreased expression of t-kallikrein may be involved in the impairment of the sweating process, vessel functionality and angiogenesis.
- PublicationOpen AccessEvaluación de la calidad del cierre de pared abdominal en modelos murinos realizada por alumnos de grado en Medicina.(Universidad de Murcia. Servicio de publicaciones, 2022) Gonzalez Lee Chong, Fatima M; Rodriguez Paz, Carlos Agustín; Moreno Ruiz, Alexia; Sánchez de Alba, Luis G; Ramírez Ramos, KevinResumen: Introducción: Se debe mejorar la docencia quirúrgica, siempre en beneficio del paciente,para que no curse con efectos adversos. Objetivo: Evaluar la calidad del cierre de pared abdominal en modelos murinos realizada por alumnos del grado en medicina. Métodos: Estudio observacional analítico, prospectivo y transversal. Se consideró conjunto universo a 55 alumnos delos cuales solo se incluyen a 35 seleccionados, a los que se les evalúo sobre modelos murinos tiporatas Wistar, tomando como variables el cierre de pared abdominal y las calificaciones según elcuestionario Anaya y Serrano. Resultados: Se evaluaron 35 cierres de pared abdominal, 23 de ellos con calidad (65.7 %) y 12, que no fueron de calidad (34.3 %). Se observa una proporción de cierres de calidad en alumnos aprobados (f=18, 78.3%) frente a no aprobados (f= 5, 21.7%) y también en los cierres de no calidad en alumnos aprobados (f=8, 66.7%) frente a no aprobados (f=4, 33.3%).Conclusión: Los cierres de pared abdominal no exitosos en modelos murinos no se relacionan con las calificaciones reprobatorias.
- PublicationOpen AccessExpression of desmosomal proteins in acantholytic squamous cell carcinoma of the skin(F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología, 2015) Jurčić, Vesna; Kukovič, Jernej; Zidar, NinaAcantholytic (adenoid) squamous cell carcinoma (ASCC) is a subtype of squamous cell carcinoma (SCC) in which neoplastic tumour cells form gland-like structures. Little is known about the pathogenetic mechanisms of ASCC. We hypothesised that they may be related to the compositon of desmosomes. We analysed the immunohistochemical expression of desmosomal proteins in 5 cases of ASCC of the skin, in comparison to 5 cases of conventional SCC of the skin. The most consistent findings were loss of desmoglein 1 (DSG 1), desmoglein 3 (DSG3), desmocollin 1 (DSC1), desmocollin 2 (DSC2), desmocollin 3 (DSC 3), and plakophilin 1 (PKP 1), and decreased expression of desmoplakin 1 (DP 1) and plakoglobin (PG). In conventional well to moderately differentiated SCC, the expression of desmosomal proteins was decreased, but membranous staining was mostly preserved with patterns similar to normal epidermis. Our results suggest that loss of desmosomal cadherins and decreased expression of desmosomal plaque proteins might be responsible for the formation of gland-like structures in ASCC. It seems that desmosomal cadherins, which correspond to the transmembrane core of desmosomes, are predominantly affected in ASCC, while DP 1 and PG, which correspond to cytoplasmic plaque of desmosomes, probably play a lesser role in maintenance of tumour cell cohesion. Our results also indicate that, in addition to previously described verrucous and spindle cell carcinoma, ASCC is another subtype of SCC with a characteristic expression pattern of desmosomal proteins.
- PublicationOpen AccessHistochemical study of lymphocystis disease in skin of gilthead seabream, Sparus aurata L.(Murcia : F. Hernández, 1998) Sarasquete, C.; Gonzalez de Canales, M.L.; Arellano, J.M.; Pérez-Prieto, S.; García-Rosado, E.; Borrego, J.J.A battery of horseradish peroxidasecorijugated lectiris (Con A, WGA arid DBA). as well as coriventional histochemical techniques (PAS. saponification, Alcian Blue pH 0.1 , 1. 2.5. chlorhydric hydrolisis. sialidasc, Bromophenol blue, Tioglycollate reduction and Ferric-ferricyanide-FeIlI) were used to study the coritent and distribution of carbohydrates, proteins and glycoconjugatc sugar residues on the skin and on the lymphocystis-infected cells of gilthead seabrcain, Spcrrus uurntcr. Variable ari-iounts of glycoproteins containing sialic acid, N-acetyl-Dglucosainine, N-acetyl-D-galactosarnine; mannose andlor glucose residues were observed in the cuticle and mucous cells of the c o r ~ o r a ls kin. tails and f ins . ~ ~ Germinative and epithelial cells of the epidermis contained glycogen. proteins. carboxylated groups, as well as glycoproteins with mannose andlor glucose and N-acetyl-U-galactosamine residues. Hyaline capsule of the mature lyniphocystis-infected cells was strongly stained with PAS, Alcian Blue (pH 0.5 and 2.5) and weakly positive with Alcian Blue (pH 1 ) . Con A reacted with the granular cytoplasm, specially around hyaline capsule. and with the basophilic intracytoplasinic inclusions developed in mature lymphocystis-infected cells of Spcrrrrs crlirntu skin. These sugar residues (niannose andlor glucose), as well as N-acetyl-Drrlucosamine andlor sialic acid and N-acetvl-D- L galactosamine were not detected in the hyaline capsule of the lyr.iphocysti5 disease.
- PublicationOpen AccessLesiones de la piel en neonatos en cuidados intensivos neonatales(Universidad de Murcia, 2018) Faraj Faria, Talita; Kamada, IvoneRESUMEN: Objetivo: Describir la ocurrencia de lesiones de la piel en recién nacidos internados en una Unidad de Cuidados Intensivos Neonatal de un hospital público de Brasilia, Distrito Federal, Brasil. Método: Estudio cuantitativo, descriptivo y de abordaje prospectivo, realizado con 104 neonatos desde septiembre hasta diciembre de 2014. El análisis estadístico descriptivo incluyó frecuencia absoluta y relativa, mediana y desvío estándar. Resultados: 42 neonatos desarrollaron lesiones de la piel, resultando 77 lesiones, siendo las más incidentes las dermatitis del pañal (15,4%), edema (15,4%), eritema (13,5%) e infiltración (12,5%), seguidas de equimosis (4,8%) y descamación (3,8%). Conclusión: Es necesaria la creación de protocolos y/o la utilización de instrumentos que miren al mantenimiento de la integridad de la piel del recién nacido, que puedan ser herramientas útiles para identificar cualquier alteración en el sistema tegumentario del neonato y, de este modo, prevenir o disminuir su incidencia y sus consecuencias.
- PublicationEmbargoMercury accumulation, structural damages, and antioxidant and immune status changes in the Gilthead Seabream (Sparus aurata L.) exposed to methylmercury(Springer, 2016-02-23) Guardiola, F. A.; Chaves-Pozo, E.; Espinosa, C.; Romero, D.; Meseguer, J.; Cuesta, A.; Esteban Abad, María Ángeles; Ciencias SociosanitariasIn aquatic systems, mercury (Hg) is an environmental contaminant that causes acute and chronic damage to multiple organs. In fish, practically all of the organic Hg found is in the form of methylmercury (MeHg), which has been associated with animal and human health problems. This study evaluates the impact of waterborne-exposure to sublethal concentrations of MeHg (10 [mu]g L^sup -1^) in gilthead seabream (Sparus aurata). Hg was seen to accumulate in liver and muscle, and histopathological damage to skin and liver was detected. Fish exposed to MeHg showed a decreased biological antioxidant potential and increased levels of the reactive oxygen molecules compared with the values found in control fish (nonexposed). Increased liver antioxidant enzyme activities (superoxide dismutase and catalase) were detected in 2 day-exposed fish with respect to the values of control fish. However, fish exposed to MeHg for 10 days showed liver antioxidant enzyme levels similar to those of the control fish but had increased hepato-somatic index and histopathological alterations in liver and skin. Serum complement levels were higher in fish exposed to MeHg for 30 days than in control fish. Moreover, head-kidney leukocyte activities increased, although only phagocytosis and peroxidase activities showed a significant increase after 10 and 30 days, respectively. The data show that 30 days of exposure to waterborne MeHg provokes more significant changes in fish than a short-term exposure of 2 or 10 days.
- PublicationOpen AccessSkin gene therapy for acquired and inherited disorders(Murcia : F. Hernández, 2006) Carretero, M.; Escámez, M.J.; Prada, F. A.; Mirones, I.; García, M.; Holguín, A.; Duarte, B.; Podhajcer, O.; Jorcano, J.L.; Larcher, F.; Del Río, M.The rapid advances associated with the Human Genome Project combined with the development of proteomics technology set the bases to face the challenge of human gene therapy. Different strategies must be evaluated based on the genetic defect to be corrected. Therefore, the re-expression of the normal counterpart should be sufficient to reverse phenotype in single-gene inherited disorders. A growing number of candidate diseases are being evaluated since the ADA deficiency was selected for the first approved human gene therapy trial (Blaese et al., 1995). To cite some of them: sickle cell anemia, hemophilia, inherited immune deficiencies, hyper-cholesterolemia and cystic fibrosis. The approach does not seem to be so straightforward when a polygenic disorder is going to be treated. Many human traits like diabetes, hypertension, inflammatory diseases and cancer, appear to be due to the combined action of several genes and environment. For instance, several wizard gene therapy strategies have recently been proposed for cancer treatment, including the stimulation of the immune system of the patient (Xue et al., 2005), the targeting of particular signalling pathways to selectively kill cancer cells (Westphal and Melchner, 2002) and the modulation of the interactions with the stroma and the vasculature (Liotta, 2001; Liotta and Kohn, 2001).
- PublicationOpen AccessSkin homeostasis during inflammation, a role for nerve growth factor(Murcia : F. Hernández, 2008) Sivilia, S.; Paradisi, M.; D’Intino, G.; Fernandez, M.; Pirondi, S.; Lorenzini, L.; Calzà, L.The skin is a neuroendocrine immune organ in which many different molecules operate in autocrineparacrine manner to guarantee tissue homeostatsis in physiological and pathophysiological conditions. In this paper we examined NGF and p75 receptor expression in the skin, during CFA induced inflammation, in a timecourse study. We also examined cutaneus innervation and proliferation, by means of immunohistochemistry and quantitative image analysis, RT-PCR and Western blot. Spontaneous and evoked pain-behavior was also measured in experimental rats. The main results can be summarized as follows: 1). a peripheral sensory neuropathy develops in this condition, as indicated by thermal hyperalgesia, thus leading to a sensory denervation of the hind-paw skin as indicated by disappearance of CGRP and PGP9.5-IR fibers; 2). NGF and p75 expression (mRNA and protein) increases in the skin (keratinocytes) in the acute phase of CFA inflammation; 3). at this stage, a higher proliferative activity is observed in the skin, as defined by the expression of cell cycle-associated protein Ki67; 4). in the long-lasting chronic phase there is a further upregulation of NFG and p75 expression in the skin; 5). trkA mRNA expression inversely correlates with p75 and NGF mRNA expression. These results suggest that CFA chronic inflammation evolves from inflammation to a small fibers sensory neuropathy and NGF seems to play a role in both events.
- PublicationEmbargoTemporal trends of inorganic elements in a common kestrel (Falco tinnunculus) population from south west Spain(Elsevier, 2021-01-18) Manzano, Javier; Mendez-Fernández, Paula; Gil-Jiménez, Esperanza; Romero, Diego; Ferrer, Miguel; Ciencias SociosanitariasHistorical variations (1966e2016) in the exposure to inorganic elements in raptors of South West Spain have been little studied. Therefore, uncertainty exists concerning whether environmental or anthropo genic inputs, as well as dietary strategy shift, may cause changes in exposure patterns. To address this gap, essential and non-essential inorganic elements were measured in Common kestrel (Falco tinnun culus) feathers from museum specimens and free-living individuals spanning a 50-year period. Moreover, stable isotopes of d13C and d15N were also measured as proxies of Common kestrel feeding ecology over time. In general, all elements showed significant increasing trends overtime, suggesting changes of in puts from local sources. Moreover, d15N signatures were correlated to the variations in Al, Cr, Cu, Fe, K, Li, Mg, Mn, Ni, P, Pb, Rb, Sr, Ti, V and Zn concentrations, suggesting trophic changes in this kestrel popu lation as well as possible biomagnification processes. Finally, some values of Cr, Mn and Pb were above the threshold concentrations of potential toxicity.
- PublicationOpen AccessThe relation between the depth of pigment disposition and men’s skin thickness, the age and tattoo locations on the body(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2023) Olszewska, Justyna; Charuta, Anna; Paziewska, Agnieszka; Wawrzyniak, Agata; Baj, Jacek; Teresiński, Grzegorz; Buszewicz, Grzegorz; Kryska, MagdalenaThe most important function of the skin is to protect the body against harmful mechanical, physical and chemical factors. Its regenerative capacity is sufficient for self-repair in the event of damage, for example, during tattooing, which can be treated as an invasive procedure introducing pigment molecules into skin layers. In the present research on tattoo pigment deposition, the structure of the dermis and epidermis was evaluated using the standard histological technique with hematoxylin and eosin staining. In addition, statistically significant differences between the depth of pigment deposition on the one hand and age, dermis and epidermis thickness and tattoo location on the other were demonstrated.
- PublicationOpen AccessThe skin injury induced by high energy dose of ultraviolet in hairless descendants of Mexican hairless dogs(Murcia : F. Hernández, 1997) Ishii, Y.; Kimura, T.; Itagaki, S.; Doi, K.Histopathological changes in the dorsal skin of hairless descendants of Mexican hairless dogs (MHDs) exposed to artificial irradiation with high energy dose ( 1 8 0 k ~ l m ~of) ultraviolet (UV) rays (UVA+B) were investigated. Macroscopically, erythema and edema were observed in the irradiated skin at 1 day after irradiation (DAI), and blister formation occurred except one dog at 2 DAI. Erythema almost disappeared at 5 DAI, and at 6 DAI, the skin recovered to almost normal state. Light microscopically, sunburn cells were observed at 1 DAI. Then intercellular edema and blister formation in the epidermis and dermal edema were evident at 2 and 3 DAI. At 6 DAI, the skin showed almost normal features except for slight epidermal thickening, but melanin granules, which were distributed in almost the whole length of the epidermis before UV irradiation, were detected only in cells which seemed to be melanocytes except one dog. Dihydroxyphenylalanine (D0PA)- positive melanocytes almost disappeared at 1 and 2 DAI, and at 6 DAI, the number of DOPA-positive melanocytes increased over the leve1 before UV irradiation. The ultrastructural features of melanocytes were characterized by vacuolated cytoplasm, decreased melanosomes, irregular-shaped nuclei and shortened dendrites at 1 DAI, and returned to normal at 6 DAI. These findings of melanocytes reflect the severity of the skin injury and support weak suntan reaction in this case. In conclusion, severe form of UV-induced skin injury seen in humans could be reproduced in hairless descendants of MHDs exposed to high energy dose of artificial UVA+B.