Browsing by Subject "Sea bream"

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    Sodium alginate as feed additive in cultured sea bream (Sparus aurata): does it modify the quality of the flesh?
    (Elsevier, 2012-05-11) García-Alcázar, A.; Abdel, I; Peso Echarri, Patricia; Frontela Saseta, María del Carmen; Santaella-Pascual, Marina; Ros Berruezo, Gaspar; Martínez Gracia, Carmen; Tecnología de Alimentos, Nutrición y Bromatología
    The objective of this study was to evaluate the effect of sodium alginate obtained from brown seaweed as a prebiotic supplement to the feed of reared sea bream (Sparus aurata). Addition of the alginate to a control diet was investigated at both concentrations 2% and 5%. Proximate composition in the flesh were not modified significantly by sodium alginate inclusion in the diet of the sea bream; however the fat and ash content in the specimens supplemented with 5% alginate were found to be significantly higher than those found in individuals who were fed the control diet. No significant differences in mineral content, fatty acid profiles, cholesterol content, texture parameters and sensory acceptability among the three studied groups. Results obtained in this study offer support for the use of alginate as a feed additive in sea bream diets since no significant effects were found in the flesh quality and characteristics of commercial size sea bream.
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    Turbot TNFα gene: molecular characterization and biological activity of the recombinant protein
    (Elsevier, 2006-04-17) Ordas, M. C.; Costa, Maria del Mar; Lopez-Castejón, Gloria; Meseguer Peñalver, J.; Figueras, Antonio; Novoa, Beatriz; Mulero Méndez, Victoriano Francisco; Roca Soler, Francisco José; Bioquímica y Biología Molecular B e Inmunología
    The tumor necrosis factor (TNF) superfamily is composed by several proteins with similar structure and functions. One of the main representatives of this family is TNF-alpha (TNFα), a proinflammatory cytokine which is produced by different immune cells and presents a wide variety of activities. Using the RACE technique, we have cloned and sequenced the turbot TNF cDNA. The analysis of its sequence showed several conserved motifs characteristic of members of the TNFα family. A phylogenetic tree constructed with different TNFs of fish and mammals grouped our sequence within the fish TNFα cluster. Therefore, the turbot TNF here studied was identified as TNFα. The complete TNFα gene was obtained by gene walking, and, similarly to the other known fish TNFα genes, presented three introns and four exons. A PCR was designed to study the turbot TNFα expression in vivo using as stimulus the bacteria Vibrio pelagius strain Hq222 and virus VHSV. The expression of the cytokine happened early after injection, and it was dependent on the pathogen injected and organ analyzed. Virus induced a higher TNFα expression, but this response was shorter in time than that induced by bacteria. In addition, TNFα expression was in general higher in kidney than in liver, as expected since the former is the haematopoietic organ of fish. The turbot recombinant TNFα (rTNFα) was obtained by IPTG induction of bacteria transformed with the pET15b-TNFα construct, and it was purified in native conditions. The recombinant protein was approximately 20 kDa in size, and its biological activity was assessed in vitro. No effect of the rTNFα neither alone nor in combination with LPS was observed on the chemiluminescence activity of turbot macrophages at any time tested. However, NO production was enhanced by the recombinant protein alone or with LPS 72 h after the addition of the treatments. Finally, turbot rTNFα was able to recruit and activate inflammatory cells when injected in gilthead seabream, although to a lesser extent than gilthead seabream rTNFα.