Browsing by Subject "Phytate"

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    Effect of Dephytinization and Follow-on Formula Addition on in Vitro Iron, Calcium, and Zinc Availability from Infant Cereals
    (American Chemical Society, 2008-04-24) Frontela Saseta, María del Carmen; Ros Berruezo, Gaspar; Martínez Gracia, Carmen; Haro Vicente, Juan Francisco; Tecnología de Alimentos, Nutrición y Bromatología
    Cereals are introduced to infants between the ages of 4 and 6 months to supplement breast milk and follow-on formula. Our objectives were to examine the content and in vitro availability of Fe, Ca, and Zn from five commercially available infant cereals mixed with water or follow-on formula before and after dephytinization. We estimated the bioaccessibility by measuring the soluble or dialyzable mineral fraction resulting from in vitro gastrointestinal digestion of the sample. For most infant cereals analyzed, dephytinization increased the in vitro availability of iron and zinc. This finding was especially dramatic among infant cereals mixed with follow-on formula rather than with water. However, the liquid used for reconstitution did not always show a significant (p < 0.05) interaction with phytase addition and in vitro mineral availability. The results of this study indicate that adding follow-on formula to infant cereals does not improve the bioaccessibility of iron, calcium, and zinc, despite the increase in mineral content it implies. Results obtained also showed that mineral solubility and dialyzability do not always follow parallel trends.
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    Effect of dephytinization on bioavailability of iron, calcium and zinc from infant cereals assessed in the Caco-2 cell model
    (Baishideng Publishing Group, 2019-04-28) Frontela Saseta, María del Carmen; Scarino,Maria Laura; Ferruzza, Simonetta; Ros Berruezo, Gaspar; Martínez Gracia, Carmen; Tecnología de Alimentos, Nutrición y Bromatología
    AIM: To test the effect of the dephytinization of three different commercial infant cereals on iron, calcium, and zinc bioavailability by estimating the uptake, retention, and transport by Caco-2 cells. METHODS: Both dephytinized (by adding an exogenous phytase) and non-dephytinized infant cereals were digested using an in vitro digestion protocol adapted to the gastrointestinal conditions of infants younger than 6 mo. Mineral cell retention, transport, and uptake from infant cereals were measured using the soluble fraction of the simulated digestion and the Caco-2 cells. RESULTS: Dephytinization of infant cereals significantly increased (P < 0.05) the cell uptake efficiency (from 0.66%-6.05% to 3.93%-13%), retention (from 6.04%-16.68% to 14.75%-20.14%) and transport efficiency (from 0.14%-2.21% to 1.47%-6.02%), of iron, and the uptake efficiency (from 5.0%-35.4% to 7.3%-41.6%) and retention (from 4.05%-20.53% to 14.45%-61.3%) of zinc, whereas calcium only cell uptake showed a significant increase (P < 0.05) after removing phytate from most of the samples analyzed. A positive relationship (P < 0.05) between mineral solubility and the cell uptake and transport efficiencies was observed. CONCLUSION: Removing phytate from infant cereals had a beneficial effect on iron and zinc bioavailability when infant cereals were reconstituted with water. Since in developing countries cereal-based complementary foods for infants are usually consumed mixed with water, exogenous phytase additions could improve the nutritional value of this weaning food
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    Phytic acid and inositol phosphates in raw flours and infant cereals: the effect of processing
    (Elsevier, 2008-03-04) Frontela Saseta, María del Carmen; García Alonso, Francisco Javier; Ros Berruezo, Gaspar; Martínez Gracia, Carmen; Tecnología de Alimentos, Nutrición y Bromatología
    Inositol phosphate modification in infant cereals was examined during industrial processing which included roasting and alphaamylase treatment. A considerable (po0.05) decrease in phytate content was observed after both treatments in all the samples analysed. However, the industrial processing observed was not sufficient to cause degradation of the phytate to achieve phytate mineral ratios optimal for mineral absorption in infant cereals. All samples analysed had a phytate/iron molar ratio 41.3, and of the 6 samples, 5 had a phytate/zinc molar ratio 414. The bioavailability of minerals is particularly important during weaning when minerals stores in infants are naturally low. Further studies are needed to evaluate the efficacy and effectiveness of phytase treatment to increase mineral bioavailability in infant foods.
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    Phytic acid content and in vitro iron, calcium and zinc bioavailability in bakery products: the effect of processing
    (Elsevier, 2011-06-14) Frontela Saseta, María del Carmen; Ros Berruezo, Gaspar; Martínez Gracia, Carmen; Tecnología de Alimentos, Nutrición y Bromatología
    Whole-grain bakery products and cereals are valuable sources of dietary fiber, vitamins, and trace elements. However, the presence of phytate, could decreases mineral bioavailability due to its chelating properties. In this study, the effect of fermentation and baking on the phytate content of different bakery products was measured by HPLC; the mineral availability in bakery products during processing was investigated by measuring solubility and dialysis, as well as the mineral uptake and transport by Caco-2 cells after in vitro digestion. Raw materials showed the highest amount of phytate, causing an important effect on the stage of processing. The solubility and dialyzability of iron increased with fermentation, meanwhile calcium and zinc showed a high variability depending on the product analyzed. After baking, the dialyzability of minerals increased with respect to the fermented dough in most cases. The highest uptake and transport efficiency of iron and calcium in cells corresponded to dough after fermentation of wheat flour with respect to the baked samples. For zinc, no differences were observed between fermented dough and after baking on uptake and transport efficiencies. This study showed that in vitro mineral availability of bakery products is influenced by the stage of processing and ingredients used.
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    The Iron and Calcium Availability and the Satiating Effect of Different Biscuits
    (MDPI, 2023-09-15) Martínez-Martínez, Antonio; Planes Muñoz, David; Ros Berruezo, Gaspar; López Nicolás, Rubén; Frontela Saseta, María del Carmen; Tecnología de Alimentos, Nutrición y Bromatología
    Biscuits are bakery products made with wheat flour. Wheat is a good source of minerals and dietary fibre, although the presence of phytate or other components could modify mineral availability. In addition, cereal-based products are usually characterised by high fibre content that can influence satiety. The objectives of this study were to evaluate both the iron and calcium availability and the satiety effect of different types of biscuits (traditional recipe vs. “Digestive”) sold in the Spanish market, identifying whether the biscuit type could have effects on these parameters. Nutritional composition and the use of the generic descriptor “Digestive” of biscuits were collected from labels. Phytate and mineral contents were also measured. All samples were previously digested by a simulated process of the gastrointestinal conditions. The satiating effect of biscuits was evaluated according to VAS questionnaires. Results indicated that the mineral content and availability were different between types of biscuits (the traditional recipe showed the highest calcium concentration, while iron was higher in the “Digestive” type). However, mineral availability showed the highest percentages for both minerals, calciumand iron, in the Maria-type samples. Regardless of the different fibre content of both types of biscuits, and despite being higher in the Digestive type than in the Maria type, the satiety measures indicated that the Maria type had more effect on the food intake control. Thus, the descriptor “Digestive¨ in biscuits does not seem to be a marker of better nutritional quality, including parameters of effects on health such as mineral availability or satiety.