Browsing by Subject "Neuroanatomy"

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    A neuroanatomical study of the feline brain using MRI and mulligan staining: functional and pathological considerations
    (Universidad de Shiraz, 2021-07-21) Díaz Martínez, Elena; Arencibia Espinosa, Alberto; Soler Laguía, Marta; Kilroy, David; Ayala Florenciano, María Dolores; Martínez Gomariz, Francisco; Ramírez Zarzosa, Gregorio José; Anatomía y Anatomía Patológica Comparadas
    Despite multiple studies describing accurate diagnoses using advanced neuroimaging techniques, low and mid-field magnetic resonance imaging (MRI) are still the most frequent scanners in veterinary clinics. To date, these studies in cats do not show a clear distinction of nerve centres in MRI data. Aims: The objective of this study is to determine the efficacy of Mulligan histological staining as a tool in facilitating the location and identification of the main structures of the feline brain in MRI. This study aims to facilitate the interpretation of MRI obtained with these types of scanners. Methods: A total of 10 feline brains were used. One specimen was used for MRI (T2 sequence using a 1.5T scanner). The other 9 brains were sectioned and stained with the three Mulligan staining techniques (Mulligan, Le Masurier and Robert). Results: The uptake of stain by the grey matter in these sections allowed the determination of the location and the limits of these nervous structures within the brain. The histological location of these structures was correlated with the MRI scans, leading to the successful identification of many small, indistinct nuclei. Conclusion: Mulligan staining is proposed as a tool that facilitates the location of nerve structures in comparison with data from the most frequently-used MRI scanners in veterinary clinics
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    A neuroanatomical study of the feline brain using MRI and mulligan staining: functional and pathological considerations
    (2021) Diaz Martínez, E.; Ayala Florenciano, María Dolores; Arencibia Espinosa, A.; Soler Laguía, Marta; Kilroy, D.; Martínez Gomariz, Francisco; Ramírez Zarzosa, Gregorio José; Anatomía y Anatomía Patológica Comparada; Facultad de Veterinaria
    Background: Despite multiple studies describing accurate diagnoses using advanced neuroimaging techniques, low and mid-field magnetic resonance imaging (MRI) are still the most frequent scanners in veterinary clinics. To date, these studies in cats do not show a clear distinction of nerve centres in MRI data. Aims: The objective of this study is to determine the efficacy of Mulligan histological staining as a tool in facilitating the location and identification of the main structures of the feline brain in MRI. This study aims to facilitate the interpretation of MRI obtained with these types of scanners. Methods: A total of 10 feline brains were used. One specimen was used for MRI (T2 sequence using a 1.5T scanner). The other 9 brains were sectioned and stained with the three Mulligan staining techniques (Mulligan, Le Masurier and Robert). Results: The uptake of stain by the grey matter in these sections allowed the determination of the location and the limits of these nervous structures within the brain. The histological location of these structures was correlated with the MRI scans, leading to the successful identification of many small, indistinct nuclei. Conclusion: Mulligan staining is proposed as a tool that facilitates the location of nerve structures in comparison with data from the most frequently-used MRI scanners in veterinary clinics.
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    Aging in the vestibular nuclear complex of the male golden hamster (Mesocricetus auratus): anatomic and morphometric study
    (Murcia : F. Hernández, 2007) Fernández, J.A.; Suárez, C.; Navarro, A.; Díaz, C.; Alvarez, J.C.; González del Rey, C.; Tolivia, J.
    To study the effects of senescence on the vestibular nuclear complex twenty brainstems from male golden hamsters between 3 and 27 months-old were used and the possible variations in the number of neurons, neuronal morphology and nuclear volume were studied. The neuron profiles were drawn with a camera lucida and Abercrombie's method was used to estimate the total number of neurons. The test of Kolmogorov-Smirnov with the correction of Lilliefors was used to evaluate the fit of our data to a normal distribution and a regression analysis was done to decide if the variation of our data with age was statistically significant. The results of the present study are relevant only for male animals and the effect of senescence could be different in female vestibular nuclear complex. Aging affects the volume of the superior and lateral vestibular nuclei, as well as the nuclear neuronal diameter of the medial vestibular nucleus, but no significant neuronal loss has been appreciated in vestibular nuclear complex related with age. During the aging process we have observed that the distribution of neurons within the vestibular nuclei of the golden hamster does not show important changes and most of their morphometric parameters do not vary significantly.
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    Dynamic patterns of colocalization of calbindin, parvalbumin and GABA in subpopulations of mouse basolateral amygdalar cells during development
    (Elsevier, 2008-01) Dávila, José Carlos; Olmos, Luis; Medina, Loreta; Guirado, Salvador; Real, Mª Angeles; Legaz Pérez, Isabel; Ciencias Sociosanitarias
    Calbindin cells represent a major interneuron subtype of the cortical/pallial regions, such as the basolateral amygdala, which are often analyzed in studies of tangential migration of interneurons from the subpallial ganglionic eminences to the pallium/cortex. However, previous evidence suggests that during development the calbindin cells may include more than one of the interneuron subtypes found in the adult pallium/cortex. Furthermore, in the adult basolateral amygdala, calbindin cells include a subpopulation of non-GABAergic (non-interneuron) cells. To better characterize these cells throughout development, in the present study we investigated the colocalization of calbindin, parvalbumin and GABA in cells of the mouse basolateral amygdala during late embryonic (E16.5) and several postnatal ages from birth until 4 weeks after birth (P0, P10 and P28). Our results indicate that CB, PV and GABA show a dynamic pattern of colocalization in cells of the mouse basolateral amygdalar nucleus throughout development. From E16.5 through P28, the majority of CB+ neurons and virtually all PV+ neurons are GABAergic. However, after P10, the percentage of GABAergic CB+ cells decline from 96% to 70%. Furthermore, while only 9% of CB+ neurons are PV+ at P10, this percentage raises to 42% at P28. At all postnatal ages studied, the majority of the PV+ cells are CB+, suggesting that PV+ interneurons develop postnatally mainly as a subpopulation within the CB+ cells of the basolateral amygdalar nucleus. These results are important for interpreting data from interneuron migration.