Browsing by Subject "MALDI MS"

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    Structural characterization of mesquite (Prosopis velutina) gum and its fractions
    (Wiley, 2008-07-31) López Franco, Yolanda L.; Calderón de la Barca, Ana María; Valdez, Miguel Ángel; Peter, Martin G.; Rinaudo, Marguerite; Chambat, Gérard; Goycoolea Valencia, Francisco Martín; Biología Celular e Histología; Facultades de la UMU::Facultad de Biología
    Structural and physicochemical characteristics of mesquite gum (from Prosopis velutina) were investigated using FT-IR spectroscopic, mass spectrometric and chromatographic methods. Four fractions (F-I, F-IIa, F-IIb and F-III) were isolated by hydrophobic interaction chromatography. The samples were characterized and analyzed for their monosaccharide and oligomers composition by high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). L-Arabinose (L-Ara) and D-galactose (D-Gal) were found as the main carbohydrate constituent residues in the polysaccharides from mesquite gum and their ratio (L-Ara/D-Gal) varied within the range 2.54 to 3.06 among the various fractions. Small amounts of D-glucose (D-Glc), D-mannose (D-Man) and D-xylose (D-Xyl) were also detected, particularly in Fractions IIa, IIb and III. Infrared spectroscopy identified polysaccharides and protein in all the samples. Data from mass spectrometry (MALDI-TOF MS) was consistent with the idea that the structure corresponding to the periphereal chains of Fraction I is predominantly a chain of pentoses attached to uronic acid.