Browsing by Subject "Hypothermia"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Publication
    Open Access
    Cold-shock proteins accumulate in centrosomes and their expression and primary cilium morphology are regulated by hypothermia and shear stress
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2024) Díaz de Cerio, María; Oliván, Sara; Ochoa, Ignacio; García Sanmartín, Josune; Martínez, Alfredo
    Primary cilia act as cellular sensors for multiple extracellular stimuli and regulate many intracellular signaling pathways in response. Here we investigate whether the cold-shock proteins (CSPs), CIRP and RBM3, are present in the primary cilia and the physiological consequences of such a relationship. R28, an immortalized retinal precursor cell line, was stained with antibodies against CIRP, RBM3, and ciliary markers. Both CSPs were found in intimate contact with the basal body of the cilium during all stages of the cell cycle, including migrating with the centrosome during mitosis. In addition, the morphological and physiological manifestations of exposing the cells to hypothermia and shear stress were investigated. Exposure to moderately cold (32°C) temperatures, the hypothermia mimetic small molecule zr17-2, or to shear stress resulted in a significant reduction in the number and length of primary cilia. In addition, shear stress induced expression of CIRP and RBM3 in a complex pattern depending on the specific protein, flow intensity, and type of flow (laminar versus oscillatory). Flow-mediated CSP overexpression was detected by qRT-PCR and confirmed by Western blot, at least for CIRP. Furthermore, analysis of public RNA Seq databases on flow experiments confirmed an increase of CIRP and RBM3 expression following exposure to shear stress in renal cell lines. In conclusion, we found that CSPs are integral components of the centrosome and that they participate in cold and shear stress sensing.
  • Thumbnail Image
    Publication
    Open Access
    Perfil de los recién nacidos de riesgo relacionados con la termorregulación en una Unidad de Cuidados Intensivos Neonatales
    (Universidad de Murcia. Servicio de publicaciones, 2021) Pinheiro Barreto, Vanessa; Rodrigues Guimarães de Aquino, Alana; Oliveira da Silva, Bárbara Coeli; Rodrigues Guimarães de Aquino, Alyne; Vasconcelos Trigueiro, Elizabeth; Rodrigues Feijão, Alexsandra
    Objetivo: Analizar el patrón de temperatura de recién nacidos de bajo peso al nacer ingresados en una Unidad de Cuidados Intensivos Neonatales. Metodología: Este es un estudio transversal y cuantitativo realizado en una Unidad de Cuidados Intensivos Neonatales en un hospital de maternidad en el noreste de Brasil. La muestra consistió en 45 niños de bajo peso al nacer, muy bajo peso al nacer o extremadamente bajo peso al nacer ingresados en la unidad. Resultados: Al ingreso se obtuvo una temperatura axilar promedio de 34.98ºC con una desviación estándar de 1.12. La tasa de hipotermia al ingreso fue considerablemente severa, por lo que en la primera hora, con seis horas y con 12 horas de hospitalización, el porcentaje de recién nacidos hipotérmicos (<36.5ºC) fue respectivamente: 93.33%, 73.33 % y 57.78%. Conclusión: Hubo fallas en los procesos cuando se trata de termorregulación, dado que casi toda la muestra llega con potencial estrés por frío.
  • Thumbnail Image
    Publication
    Open Access
    Treatment and new progress of neonatal hypoxic-ischemic brain damage
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Yang, Lijun; Zhao, Hehua; Cui, Hong
    Neonatal hypoxic ischemia (HI) results in different extents of brain damage, and immature brain tissue is particularly sensitive to the stimulation of HI. Hypoxic-ischemic brain damage (HIBD) is a common and serious nervous system disease in neonates, for both full-term infants and preterm infants, and is one of the main causes of neonatal death. The surviving infants are often associated with cerebral palsy, mental retardation, and other sequelae, which severely affect quality of life. For term infants, hypoxia and ischemia mainly affect gray matter, whereas in preterm infants, the white matter. However, up to now, inadequate standards and specific measures that can be used to treat hypoxic-ischemic brain injury are available. Recently, in addition to supportive therapy and symptomatic treatment, research on the treatment of hypoxic-ischemic brain injury has focused on the following aspects: hypothermia therapy, stem cell therapy, neuroprotective agents, ibuprofen, and combination therapy. In this review, we will summarize the treatment of HIBD and make suggestions for the future treatment direction