Browsing by Subject "Gilthead seabream"
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- PublicationOpen Access17α-Ethynylestradiol alters the peritoneal immune response of gilthead seabream(Elsevier, 2017) Gómez González, Nuria Esther; Cabas, Isabel; Rodenas, María del Carmen; Arizcun, Marta; Mulero Méndez, Victoriano Francisco; García Ayala, Alfonsa; Biología Celular e Histología17α-Ethynylestradiol (EE2), a synthetic estrogen used in most oral contraceptives pills and hormone replacement therapies, is found in many water bodies, where it can modulate the fish immune response. EE2 acts as an endocrine disruptor in gilthead seabream, Sparus aurata L., a marine teleost fish of great economic value in Mediterranean aquaculture, as it induces hepatic vitellogenin gene (vtg) expression. Moreover, EE2 also alters the capacity of gilthead seabream to appropriately respond to infection although it does not behave as an immunosuppressor. Nevertheless, these previous studies have mainly focused on the head kidney leukocytes and no information exists on peritoneal leukocytes, including mast cells. In the present work, juvenile gilthead seabream fish were fed a pellet diet supplemented with EE2 for 76 days and intraperitoneally injected with hemocyanin plus imject alum adjuvant at the end of EE2 treatment and 92 days later, and the peritoneal immune response was analyzed. EE2 supplementation induced vtg expression but returned to basal levels by 3 months post-treatment. Interestingly, gilthead seabream peritoneal leukocytes express the genes encoding for the nuclear estrogen receptor α and the G protein-coupled estrogen receptor 1 and the dietary intake of EE2 induced these expression. Moreover, EE2 induced an inflammatory response in the peritoneal cavity in unvaccinated fish, which was largely maintained for several months after the cessation of the treatment. However, the impact of EE2 in vaccinated fish was rather minor and transient. Taken together, the study provides fresh information about endocrine immune disruption, focusing on peritoneal leukocytes.
- PublicationEmbargoEffect of dietary supplementation of probiotics and palm fruits extracts on the antioxidant enzyme gene expression in the mucosae of gilthead seabream (Sparus aurata L.)(Elsevier, 2014-07) Esteban Abad, María Ángeles; Cordero, H; Martínez Tomé, Magdalena; Jiménez Monreal, A.M; Bakhrouf, A; Mahdhi, A; Tecnología de Alimentos, Nutrición y BromatologíaAntioxidant activity is particularly important, since oxidation is an unavoidable reaction in all living bodies. At present, natural antioxidants to be used on food as an alternative to synthetic ones are being sought. Gilthead seabream (Sparus aurata L.) specimens were fed for 4 weeks with diets enriched with bacterial probiotics (Shewanella putrefaciens Pdp11 and Bacillus sp), single or in combination with Tunisian dates palm fruit extracts. The expression of the main antioxidant enzyme genes (superoxide dismutase, catalase and glutathione reductase) in the mucosae (gut, skin and gill) was evaluated after 2 and 4 weeks. Previously, free radical scavenging and several antioxidant assays were developed to know the antioxidant properties present on the palm fruits extracts. The results demonstrated that experimental diets alter the expression of the studied antioxidant genes, primarily in the gill and skin. Furthermore, the tested probiotics and mainly, the aqueous date palm fruits extracts had significant antioxidant properties based on their protective effect against the levels of intracellular reactive oxygen species, especially when administering during 4 weeks. For this reason, probiotics and date palm fruit extracts may serve as good natural antioxidants and could potentially be considered as a functional food ingredient for fish in farms.
PublicationOpen AccessErythrocyte phagocytosis in gilthead seabream (Sparus aurata) and European sea bass (Dicentrarchus labrax).(2024-11-04) Campos Sánchez, Jose Carlos; Guardiola, Francisco A.; Meseguer, José; Esteban Abad, María Ángeles; Biología celular e Histología- PublicationOpen AccessFeeding time synchronises daily rhythms of behaviour and digestive physiology in gilthead seabream (Sparus aurata)(Elsevier, 2010) Montoya, Ander; López Olmeda, José Fernando; Yúfera, Manuel; Sánchez Muros, María José; Sánchez Vázquez, Francisco Javier; FisiologíaFeeding cycles entrain biological rhythms, which enables animals to anticipate feeding times and so maximizes food utilization. In this article the effect of mealtime on locomotor activity, blood glucose, gastric pH and digestive enzymes was studied in two groups of seabream (Sparus aurata): one group received a single daily meal at random times either during the light or the dark (random feeding, RF), whereas the other group received the meal during the light period every day at the same time (periodic feeding, PF). PF fish showed strong synchronisation of locomotor activity to the light phase (97.9 ± 0.2% of their total daily activity during daytime). In addition, the locomotor activity rhythm of PF fish showed a statistically significant daily rhythm (p < 0.05) for a period of 24 h, whereas RF fish did not display a statistically significant rhythm. Blood glucose levels were higher in RF fish during the 8 h following feeding. Gastric pH showed a postprandial decrease in both groups, but RF fish showed a lower daily average value (4.31 ± 0.21 compared with 5.52 ± 0.20). Amylase and alkaline protease activity increased some hours before mealtime in PF fish, whereas amylase activity increased 1 h after feeding and alkaline protease showed no statistically significant differences in RF fish. Acid protease activity showed no statistically significant differences in any group. Taken together, these results demonstrate that altering the feeding time affects the physiology and behaviour of seabream, which have the capacity to prepare themselves for a forthcoming meal.
- PublicationOpen AccessFunctionalized Nanoplastics (NPs) Increase the Toxicity of Metals in Fish Cell Lines(MDPI (Basel, Switzerland), 2021) González Fernández, Carmen; Díaz Baños, F. Guillermo; Esteban Abad, María Ángeles; Cuesta, Alberto; Química FísicaNanoplastics (NPs) are one of the most abundant environment‐threatening nanomaterials on the market. The objective of this study was to determine in vitro if functionalized NPs are cytotoxic by themselves or increase the toxicity of metals. For that, we used 50 nm polystyrene nanoparticles with distinct surface functionalization (pristine, PS‐Plain; carboxylic, PS‐COOH; and amino PS‐NH2) alone or combined with the metals arsenic (As) and methylmercury (MeHg), which possess an environmental risk to marine life. As test model, we chose a brain‐derived cell line (SaB‐1) from gilthead seabream (Sparus aurata), one of the most commercial fish species in the Mediterranean. First, only the PS‐NH2 NPs were toxic to SaB‐1 cells. NPs seem to be internalized into the cells but they showed little alteration in the transcription of genes related to oxidative stress (nrf2, cat, gr, gsta), cellular protection against metals (mta) or apoptosis (bcl2, bax). However, NPs, mainly PSCOOH and PS‐NH2, significantly increased the toxicity of both metals. Since the coexistence of NPs and other pollutants in the aquatic environment is inevitable, our results reveal that the combined effect of NPs with the rest of pollutants deserves more attention.
- PublicationOpen AccessLight and feeding entrainment of the molecular circadian clock in a marine teleost (Sparus aurata)(Taylor and Francis Group, 2013-05-20) Vera Andujar, Luisa María; Negrini, P.; Zagatti, C.; Frigato, E.; Sánchez-Vázquez, F.J.; Bertolucci, C.; FisiologíaDaily light and feeding cycles act as powerful synchronisers of circadian rhythmicity. Ultimately, these external cues entrain the expression of clock genes, which generate daily rhythmic behavioural and physiological responses in vertebrates. In the present study, we investigated clock genes in a marine teleost (gilthead seabream). Partial cDNAs sequences of key elements from both positive (Bmal1, Clock) and negative (Per2, Cry1) regulatory loops were cloned before studying how feeding time affects the daily rhythms of locomotor activity and clock gene expression in the central (brain) and peripheral (liver) oscillators. To this end, all fish were kept under a light-dark (LD) cycle and were divided into three experimental groups, depending on the time of their daily meal: mid-light (ML), middarkness (MD) or at random times (RD). Finally, the existence of circadian control on gene expression was investigated in the absence of external cues (DD + RD). The behavioural results showed that seabream fed at ML or RD displayed a diurnal activity pattern (>91% of activity during the day), whereas fish fed at MD were nocturnal (89% of activity during the night). Moreover, seabream subjected to regular feeding cycles (ML and MD groups) showed food anticipatory activity (FAA). Regardless of the mealtime the daily rhythm of clock gene expression in the brain peaked close to the light-dark transition in the case of Bmal1 and Clock, and at the beginning of the light phase in the case of Per2 and Cry1, showing the existence of phase delay between the positive and negative elements of the molecular clock. In the liver, however, the acrophases of the daily rhythms differed depending on the feeding regime: the maximum expression of Bmal1 and Clock in the ML and RD groups was in antiphase to the expression pattern observed in the fish fed at MD.Under constant conditions (DD + RD), Per2 and Cry1 showed circadian rhythmicity in the brain, whereas Bmal1, Clock and Per2 did in the liver. Our results indicate that the seabream clock gene expression is endogenously controlled and in liver it is strongly entrained by food signals, rather than by the LD cycle, and that scheduled feeding can shift the phase of the daily rhythm of clock gene expression in a peripheral organ (liver) without changing the phase of these rhythms in a central oscillator (brain), suggesting uncoupling of the light-entrainable oscillator (LEO) from the food-entrainable oscillator (FEO). These findings provide the basis and new tools for improving our knowledge of the circadian system and entraining pathways of this fish species, which is of great interest for the Mediterranean aquaculture.