Browsing by Subject "Fish cell lines"

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    Cellular responses to electromagnetic fields and ultraviolet radiation: an in vitro study on microalgae and fish cell lines
    (Elsevier, 2026) Ortiz García, María; Terrer Marcilla de Teruel Moctezuma, José María; García Gamuz, José Antonio; Esteban Abad, María de los Ángeles; Aboal Sanjurjo, Marina; Biología Celular e Histología; María Ángeles Esteban Abad; Facultades de la UMU::Facultad de Biología
    Background The molecular effects involved in the cellular response to non-ioninzing electromagnetic radiation on marine and freshwater microalgae and fish cell lines are incompletely understood. Objectives The direct impact of non-ionizing electromagnetic radiation on marine and freshwater microalgae and fish cell lines was investigated. Methods Six microalgae species from different taxonomic groups were exposed to extremely low-frequency electromagnetic fields (ELF-EMF) for five days. Four fish cell lines (SAF-1, DLB-1, DLEC, and Fugu eye) were exposed to ultraviolet (UV) radiation, with SAF-1 demonstrating the highest sensitivity. In addition, three commercial antioxidants (2101, 3600, and 4004) were tested for their possible radioprotective effects. Results Although no statistically significant differences in cell density were observed, certain taxa (Chlorotylium sp., Mayamea sp., and Scenedesmus sp.) exhibited a declining trend compared to the controls, suggesting species-specific sensitivity to ELF-EMF. Principal component analysis revealed significant differences in physiological responses, particularly in photosynthetic variables such as electron transport rate and dissolved carbon. The total antioxidant capacity did not differ significantly between the control and exposed microalgae, and Chlorotylium sp. showed the highest basal levels. The photosystem II productivity index remained largely unaffected by ELF-EMF exposure, except for an increase in irradiated Scenedesmus sp. Low antioxidant concentrations (≤125 µg/ml) and short incubation times (6 h) of 2101 and 3600 induced proliferation or increased cell viability, particularly under irradiation. However, prolonged incubation time (24 h) or high antioxidant concentrations (≥250 µg/ml) resulted in significant cytotoxicity in all cell lines.
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    Effects of cantharidin on fish erythrocytes, tumour cell lines, and marine pathogenic bacteria
    Campos-Sánchez, José Carlos; Esteban Abad, María de los Ángeles; Guardiola Abellán, Francisco Antonio; Biología Celular e Histología
    Cantharidin, a toxic monoterpene secreted by blister beetles, has long been used in traditional Chinese and modern medicine for its unique properties. However, despite its widespread use, its effects on fish have not been studied in depth. The aim of this study was to evaluate the potential therapeutic applications of cantharidin in fish by examining its antioxidant, hemoagglutinating, hemolytic and cytotoxic activities at different concentrations (0, 0.625, 1.25, 2.5, 5 and 10 μg mL-1) in three different cell lines. In addition, the study explored the bactericidal and bacteriostatic properties of cantharidin against various fish pathogenic bacteria. The results revealed that there were no significant differences in antioxidant, hemagglutinating or hemolytic activities between the different concentrations of cantharidin tested. However, the study found that cantharidin exhibited dose- and time-dependent cytotoxicity in sea bream (Sparus aurata) erythrocytes and in SAF-1, PLHC-1 and Hela cell lines, resulting in morphological changes indicative of apoptosis. Interestingly, the highest dose of cantharidin tested demonstrated potent bactericidal activity against four marine fish opportunistic bacteria, including Vibrio harveyi, V. anguillarum, Photobacterium damselae and Tenacibaculum maritimum, but no statistically significant changes in bacteriostatic activity were observed against any of the bacteria tested. Overall, these results provide valuable information on the potential therapeutic applications of cantharidin in fish aquaculture. Further research is needed to fully understand the mechanisms of action and to explore possible preventive uses of cantharidin in fish.
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    In vitro effects of a natural marine algae polysaccharide (λ-carrageenin) on seabream erythrocytes, tumour cell lines and marine bacterial pathogens
    Campos-Sánchez, José Carlos; Esteban Abad, María de los Ángeles; Guardiola Abellán, Francisco Antonio; Biología Celular e Histología
    Carrageenin is a high molecular weight sulphated mucopolysaccharide used to induce experimental inflammation in mammals. In addition, it possesses a wide variety of properties that have not yet been studied in fish. This study evaluated the hemagglutinating, hemolytic, cytotoxic, and antibacterial activities of λ-carrageenin. The results showed that λ-carrageenin has hemagglutinating and hemolytic activities on gilthead seabream erythrocytes, which were dose and time-dependent during the first 6 hours of incubation. No significant effects on the haemolytic activity of erythrocytes were observed after incubation for 12 or 24 h with λ-carrageenin. The PLHC-1 cell line showed significant increases in cytotoxic activity after 6 or 12 hours of incubation compared with control cells, and the highest doses of λ-carrageenin caused cytotoxicity in PLHC-1 cells after 24 h of incubation. The morphology of PLHC1 cells incubated with the highest doses of λ-carrageenin for 12 or 24 h showed obvious cell death changes compared with control cells. Interestingly, no significant variations in cytotoxic activity were observed in SAF-1 or HeLa cell lines after incubation with λ-carrageenin. λ-carrageenin showed significant dose-dependent bactericidal activity against Photobacterium damselae but had no significant effect on the bactericidal activity of Vibrio harveyi, V. anguillarum, and Tenacibaculum maritimum. The study suggests that λ-carrageenin has potential applications in aquaculture and aquatic pharmaceutical industries as a hemagglutinating, hemolytic, and antibacterial agent.