Browsing by Subject "Degree of acetylation"

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    An infrared investigation in relation with chitin and chitosan characterization
    (Elsevier, 2001-01-12) Brugnerotto, J.; Lizardi, J.; Argüelles Monal, Waldo; Desbrières, J.; Rinaudo, Marguerite; Goycoolea Valencia, Francisco Martín; Biología Celular e Histología
    The use of infrared spectroscopy for characterization of the composition of chitin and chitosan covering the entire range of degree of acetylation (DA) and a wide variety of raw materials is examined further. The ratio of absorbance bands selected was calibrated using 1H liquid and 13C CP-MAS solid-state NMR as absolute techniques. IR spectra of the structural units of these polymers validated the choice of baselines and characteristic bands. The bands at 1650 and 1320 cm-1 were chosen to measure the DA. As internal reference, the intensities at 3450 and 1420 cm-1 were evaluated. The absorption band ratios involving the reference at 3450 cm-1 had poorer fit. The absorption ratio A1320/A1420 shows superior agreement between the absolute and estimated DA-values (DA% = 31.92A1320/A1420 - 12.20; r = 0.990)