Datos de Investigación

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Open Access
A new approach to study inflammation in fish: Serum proteinogram analysis in gilthead seabream (Sparus aurata) injected with λ-carrageenan
Campos Sánchez, Jose Carlos; Esteban Abad, María Ángeles; Guardiola, Francisco A.; Biología celular e Histología
Open Access
Bioinformatic and gene expression analysis of the somatostatin/cortistatin gene family in the gilthead seabream (Sparus aurata).
Campos Sánchez, Jose Carlos; Serna‑Duque, Jhon A.; Guardiola, Francisco A.; Cuesta, Alberto; Esteban Abad, María Ángeles; Biología celular e Histología
Open Access
Data of the work entitled: Ability of short-chain fatty acids to reduce inflammation and attract leucocytes to the inflamed skin of gilthead seabream (Sparus aurata L.)
Albaladejo-Riad, Nora; El qendouci, Mouna; Cuesta, Alberto; Esteban Abad, María Ángeles; Biología celular e Histología
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Data set of Novel hepcidin genes in Gilthead Seabream: implications for immune response and iron metabolism
(2025-04-24) García Navarro, Laura; Serna Duque, Jhon; Cuesta Peñafiel, Alberto; Esteban Abad, Mª Ángeles; Biología celular e Histología
Results of Synteny and phylogeny analysis of hamp2.0 and hamp2.15 in the class Actinopterygii, Bactericidal activity of HAMP2α or HAMP2Ω, Cytotoxicity activity of HAMP2α or HAMP2Ω. And a QuantStudio™ 5 Real-Time PCR System (Applied Biosystems).
Open Access
Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries
(Elsevier, ) Albaladejo-Riad, Nora; Espinosa-Ruiz, Cristóbal; Esteban Abad, María Ángeles; Biología celular e Histología
Silk fibroin microparticles (SF) were administered in the diet of gilthead seabream with or without experimental skin wounds to study the effects on cellular immunity and liver. A commercial diet was enriched with different SF contents: 0, 50 and 100 mg kg-1 (control, SF50 and SF100 diet, respectively). The animals were fed for 30 days and half of them were sampled. Similar experimental wounds were then performed to the rest of fish and they continued to be fed the same diet. At 7 days post-wounding, samples were taken from the wounded fish. Cellular immunity was studied on head kidney leucocytes (phagocytosis, respiratory and peroxidase content) and liver status (histological study and gene expression) were studied. Our results showed that experimental wounds affect both cellular immunity (by decreasing leucocyte respiratory burst and peroxidase activity) and altered liver histology (by inducing vascularization and congestion of blood vessels). In addition, it affects the expression of genes indicative of oxidative stress, endoplasmic reticulum stress and apoptosis. More specifically, it was the highest dose of silk fibroin (SF100) that significantly increased the phagocytic capacity of leucocytes as well as vascularization in the liver. In addition, increased expression of antioxidant genes (cat and gsr) and decreased expression of genes related to reticulum endoplasmic stress (grp94 and grp170) and apoptosis (nos and jnk) were detected in these fish fed with SF100 and wounded. In conclusion, fed fish with SF100 had many beneficial effects as cellular immunostimulant and hepatoprotection in wounded fish. Its use could be of great interest for stress management in farmed fish conditions.
Open Access
Effects of cantharidin on fish erythrocytes, tumour cell lines, and marine pathogenic bacteria
Campos-Sánchez, José Carlos; Guardiola, Francisco A.; Esteban Abad, María Ángeles; Biología celular e Histología
Cantharidin, a toxic monoterpene secreted by blister beetles, has long been used in traditional Chinese and modern medicine for its unique properties. However, despite its widespread use, its effects on fish have not been studied in depth. The aim of this study was to evaluate the potential therapeutic applications of cantharidin in fish by examining its antioxidant, hemoagglutinating, hemolytic and cytotoxic activities at different concentrations (0, 0.625, 1.25, 2.5, 5 and 10 μg mL-1) in three different cell lines. In addition, the study explored the bactericidal and bacteriostatic properties of cantharidin against various fish pathogenic bacteria. The results revealed that there were no significant differences in antioxidant, hemagglutinating or hemolytic activities between the different concentrations of cantharidin tested. However, the study found that cantharidin exhibited dose- and time-dependent cytotoxicity in sea bream (Sparus aurata) erythrocytes and in SAF-1, PLHC-1 and Hela cell lines, resulting in morphological changes indicative of apoptosis. Interestingly, the highest dose of cantharidin tested demonstrated potent bactericidal activity against four marine fish opportunistic bacteria, including Vibrio harveyi, V. anguillarum, Photobacterium damselae and Tenacibaculum maritimum, but no statistically significant changes in bacteriostatic activity were observed against any of the bacteria tested. Overall, these results provide valuable information on the potential therapeutic applications of cantharidin in fish aquaculture. Further research is needed to fully understand the mechanisms of action and to explore possible preventive uses of cantharidin in fish.
Open Access
Erythrocyte phagocytosis in gilthead seabream (Sparus aurata) and European sea bass (Dicentrarchus labrax).
(2024-11-04) Campos Sánchez, Jose Carlos; Guardiola, Francisco A.; Meseguer, José; Esteban Abad, María Ángeles; Biología celular e Histología
Open Access
Gene expression raw data of the work entitlled: "evolution of a gilthead sea bream (Sparus aurata L.) caudal fin skin explant"
Albaladejo-Riad, Nora; Esteban Abad, María Ángeles; Cuesta, Alberto; García-Álvarez, Miguel Ángel; Marín-Parra, Claudia; Biología celular e Histología
Open Access
In vitro effects of a natural marine algae polysaccharide (λ-carrageenin) on seabream erythrocytes, tumour cell lines and marine bacterial pathogens
Campos-Sánchez, José Carlos; Guardiola, Francisco A.; Esteban Abad, María Ángeles; Biología celular e Histología
Carrageenin is a high molecular weight sulphated mucopolysaccharide used to induce experimental inflammation in mammals. In addition, it possesses a wide variety of properties that have not yet been studied in fish. This study evaluated the hemagglutinating, hemolytic, cytotoxic, and antibacterial activities of λ-carrageenin. The results showed that λ-carrageenin has hemagglutinating and hemolytic activities on gilthead seabream erythrocytes, which were dose and time-dependent during the first 6 hours of incubation. No significant effects on the haemolytic activity of erythrocytes were observed after incubation for 12 or 24 h with λ-carrageenin. The PLHC-1 cell line showed significant increases in cytotoxic activity after 6 or 12 hours of incubation compared with control cells, and the highest doses of λ-carrageenin caused cytotoxicity in PLHC-1 cells after 24 h of incubation. The morphology of PLHC1 cells incubated with the highest doses of λ-carrageenin for 12 or 24 h showed obvious cell death changes compared with control cells. Interestingly, no significant variations in cytotoxic activity were observed in SAF-1 or HeLa cell lines after incubation with λ-carrageenin. λ-carrageenin showed significant dose-dependent bactericidal activity against Photobacterium damselae but had no significant effect on the bactericidal activity of Vibrio harveyi, V. anguillarum, and Tenacibaculum maritimum. The study suggests that λ-carrageenin has potential applications in aquaculture and aquatic pharmaceutical industries as a hemagglutinating, hemolytic, and antibacterial agent.
Open Access
In vitro immune-depression and anti-inflammatory activities of cantharidin on gilthead seabream (Sparus aurata) leucocytes activated by λ-carrageenan
Campos-Sánchez, Jose Carlos; Guardiola, Francisco A.; Esteban Abad, María Ángeles; Biología celular e Histología
Cantharidin is a natural compound with known therapeutic applications in humans. However, its properties have hardly been studied in fish. The aim of this study was to investigate the in vitro effects of cantharidin on gilthead seabream (Sparus aurata) head kidney leucocytes (HKL) stimulated with λ-carrageenan. Thus, HKLs were incubated for 24 h with cantharidin (0, 2.5 and 5 μg mL-1) and λ-carrageenan (0 and 1,000 μg mL-1) and cell viability and morphology, immunological parameters and several selected genes related to inflammation and apoptosis were evaluated. The results showed that cantharidin only decreased HKL viability when incubated with the highest dose (5 μg mL-1) and in combination with λ-carrageenan. As for the immunological parameters studied, cantharidin increased the peroxidase activity of HKLs only when incubated in combination with λ-carrageenan, but inhibited the respiratory burst and phagocytic activities even after being previously increased by λ-carrageenan. Furthermore, cantharidin induced morphological changes in HKLs with apoptotic and vacuolization signs that were enhanced when incubated in combination with λ-carrageenan. Considering the analysis of gene expression in HKLs, although λ-carrageenan up-regulated the expression of the proinflammatory gene il1b, cantharidin down-regulated its expression in HKLs exposed to both λ-carrageenan and cantharidin. In addition, cantharidin up-regulated casp3 and casp9 expression, which was down-regulated in HKLs incubated with both molecules. This combination also up-regulated casp1 expression. The present results provide detailed insight into the immune-depressive and anti-inflammatory properties of cantharidin on immune cells, which could be of interest to the aquaculture sector.
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Metabolomic profiling of the serological response to a hepcidin 1 injection in gilthead seabream (Sparus aurata)
García Navarro, Laura; Esteban Abad, María Ángeles; Esteban Abad, María Ángeles; Biología celular e Histología
Datos obtenidos mediante HILIC y RP con ESI positiva y negativa.
Open Access
Mitochondrial metabolism characterization of four different fish cell lines
Espinosa-Ruiz, Cristóbal; Mayor-Lafuente, Javier; Esteban Abad, María Ángeles; Biología celular e Histología
Different cell lines (SAF-1, DLB-1, PLHC-1 and FuB-1) mitro stress (OCR and ECAR) raw data.
Open Access
Participation of hepcidins in the inflammatory response triggers by λ-carrageenin in gilthead seabream (Sparus aurata)
Campos-Sánchez, José Carlos; Serna-Duque, Jhon A.; Alburquerque, Carmen; Guardiola, Francisco A.; Esteban Abad, María Ángeles; Biología celular e Histología
The role of hepcidins, antimicrobial peptides involved in iron metabolism, immunity, and inflammation is studied. First, gilthead seabream (Sparus aurata L.) head-kidney leucocytes (HKLs) were incubated with λ-carrageenin to study the expression of hepcidin and iron metabolism-related genes. The expression of most of the genes studied was up-regulated, whereas the ferroportin gene (slc40a) was down-regulated in HKLs incubated with λ-carrageenin. In the second part of the study, seabream specimens were injected with λ-carrageenin or buffer (control). The expression of the same genes was evaluated in the head kidney, liver, and skin at different time points after injection. The expression of Hamp1m, ferritin b and ferroportin genes (hamp1, fthb, and slc40a) was up-regulated in the head kidney of fish from the λ-carrageenin group, while the expression of Hamp2C and Hamp2E genes (hamp2.3 and hamp2.7) was down-regulated. In the liver, the expression of hamp1, ferritin a (ftha), slc40a, Hamp2J and Hamp2D (hamp2.5/6) genes was down-regulated in the λ-carrageenin group. In the skin, the expression of hamp1 and (Hamp2A Hamp2C) hamp2.1/3/4 genes was up-regulated in the λ-carrageenin group. A bioinformatic analysis was performed to predict the presence of transcription factor binding sites in the promoter region of hepcidins. Structural and physicochemical variations were found in the different mature hepcidin peptides. This study sheds light on the poorly understood roles of hepcidins in fish. The results provide insight into the regulatory mechanisms of inflammation in fish and could contribute to the development of new strategies for treat inflammation in farm animals.
Open Access
Serum proteinogram of gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax) as a new useful approach for detecting loss of haemostasis
Campos-Sánchez, Jose Carlos; Guardiola, Francisco A.; Esteban Abad, María Ángeles; Biología celular e Histología
Open Access
Similarities between the lipid profiles of skin and liver of European Sea Bass (Dicentrarchus labrax) fed diets with different lipid content
(Springer, ) Espinosa-Ruiz, Cristóbal; González-Silvera, Daniel; Esteban Abad, María Ángeles; Biología celular e Histología
The specific fatty acid profile of fish can vary depending on species, age, diet and other factors. However, in general, fish are generally rich in long-chain polyunsaturated fatty acids (LC-PUFA), such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Liver, skin, meat and fish mucus can contain different levels and types of fatty acids. In fact, each of these tissues can provide valuable information about the fatty acid profile of the whole fish. In order to establish a non-invasive method to assess the lipid profile of fish tissues, twenty-four European sea bass (Dicentrarchus labrax) were fed two different diets [containing different PUFAs profiles] for 30 days. Samples of cutaneous mucus, skin, flesh and liver were then obtained and their fatty acid profile was analyzed by gas chromatography. Multidimensional scaling (MDS), SIMPER procedure (similarity percentages) and permutation test (PERMANOVA) were performed. The results indicate that there are similarities in the fatty lipid profile of skin and liver, as well as between skin and liver mucus. Relationships have been observed between the fatty acid compositions of the different fish tissues, in particular between liver and skin, where linoleic acid (18:2n-6) is the most significant, which would allow the skin to be used as a proxy to infer the hepatic lipid profile, without the need to sacrifice the animals
Open Access
Skin mucus metabolomics provides insights into the interplay between diet and wound in gilthead seabream (Sparus aurata)
(Frontiers, ) Albaladejo-Riad, Nora; Espinosa-Ruiz, Cristóbal; Esteban Abad, María Ángeles; Lazado, Carlos C.; Biología celular e Histología
Ultra Performance Liquid Chromatography coupled with a high-resolution quadrupole-orbitrap mass spectrometry DATA
Open Access
Use of carrageenan in the screening of natural anti-inflammatory molecules in fish: in vitro effects of Chiliadenus glutinosus extracts
Campos-Sánchez, Jose Carlos; Guardiola, Francisco A.; Esteban Abad, María Ángeles; Biología celular e Histología
Carrageenan, a high molecular weight sulphated polysaccharide, has been used in the screening of anti-inflammatory molecules in mammals. In this sense, Chiliadenus glutinosus (L.) Fourr., an endemic species of plant has been reported in the Spanish popular medicine due to their anti-inflammatory properties in humans. However, their properties have poorly been studied in fish. In this assay, different concentrations (0, 0.001, 0.01, 0.125, 0.25, 0.5 and 1 mg mL-1) of aqueous, ethanolic and methanolic extracts of this plant were elaborated, and the antioxidant, bactericidal (against four marine fish pathogen) and cytotoxic activity, as well as several immunological parameters in head-kidney leucocytes (HKLs) of gilthead seabream (Sparus aurata) were evaluated in a first experiment to determinate the extract with the best properties. Our results showed a dose-dependent antioxidant activity of each plant extract increasing with the concentration, a species- and extract-specific bactericidal activity, as well as a cytotoxic activity against the PLHC1 tumour cell line by the ethanolic and methanolic extracts. However, taking into account the cytotoxic effects of these two extracts on HKLs and the strong immunosuppressive effects on the immunological parameters assayed (peroxidase, respiratory burst activity and phagocytic capacity), a second experiment was developed by using only adjusted doses of the aqueous extract (0, 0.25 and 0.5 mg mL-1), in which immune parameters, cell morphology and inflammatory-related genes were studied in HKLs stimulated with λ-carrageenan. The present results offer a detailed view of the biological effects of C. glutinosus extracts, which could be interesting to the aquaculture sector due to their possible anti-inflammatory effects.

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