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Título: | Liver growth factor antifibrotic activity in vivo is associated with a decrease in activation of hepatic stellate cells |
Fecha de publicación: | 2009 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | 576 - Biología celular y subcelular. Citología |
Palabras clave: | Fibrosis Myofibroblasts |
Resumen: | The antifibrotic activity of Liver Growth Factor (LGF), a liver mitogen, was previously demonstrated in several models of rat liver fibrosis and even in extrahepatic sites, such as carotid artery in hypertensive rats and rat CdCl2-induced lung fibrosis. In the present study, we have attempted to examine in depth its mechanism of antifibrotic action in bile duct-ligated (BDL) rats, with special emphasis on its activity in fibrogenic liver cells. BDL rats received either LGF 9 μg/week for 2 or 3 weeks (BDL+LGF, n=20/group) or saline (BDL+S, n=20/group), at times 0, week 2, or week 5 after operation. Groups were compared in terms of liver a- smooth muscle actin (SMA) content (western blotting and immunohistochemistry), hepatic apoptosis, liver desmin content (western blotting), and serum endothelin-1 (ELISA). LGF produced a marked decrease in liver a-SMA content compared with saline-injected rats, especially evident at longer times (5w and 8w; p<0.05), accompanied by a decrease in hepatic a-SMA+ cells. This decrease was not due to the killing of activated hepatic stellate cells (HSC) or myofibroblasts by LGF, since there was a slight decrease in hepatic apoptosis that was more evident at 2w (p<0.05). Moreover, LGF did not seem to influence HSC proliferation, as shown by measuring liver desmin content. The antifibrotic activity exerted by LGF seems to be closely related to a modulation of the activation state of fibrogenic liver cells (activated HSC and myofibroblasts) in BDL rats. |
Autor/es principal/es: | Díaz-Gil, Juan J. García-Monzón, Carmelo Rúa, Carmen Martín Sanz, Paloma Cereceda, Rosa M. Miquilena-Colina, María E. Machín, Celia Fernández Martínez, Amalia García Cañero, Rafael |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/36009 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 7 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.24, nº4 (2009) |
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Liver growth factor antifibrotic activity in vivo is associated with a.pdf | 1,47 MB | Adobe PDF | Visualizar/Abrir |
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