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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Lai, C.C. | - |
dc.contributor.author | Liu, T. J. | - |
dc.contributor.author | Ho, L. K. | - |
dc.contributor.author | Don, M. J. | - |
dc.contributor.author | Chau, Y. P. | - |
dc.date.accessioned | 2020-05-22T15:02:47Z | - |
dc.date.available | 2020-05-22T15:02:47Z | - |
dc.date.issued | 1998 | - |
dc.identifier.citation | Histology and histopathology, Vol. 13, nº1 (1998) | es |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.uri | http://hdl.handle.net/10201/92548 | - |
dc.description.abstract | In present study we studied the cytotoxic effects of B-lapachone, a potent anticancer drug, on the human hepatoma cell line (HepA2) under serum-free condition. Most cells died after 2 ,uM B-Iapachone addition at 48 hours. No apoptotic characteristics of DNA ladder was documented by agarose DNA electrophoresis. The blockage of cell cycle at S phase and unscheduled DNA synthesis were demonstrated by flow cytometric analysis and anti-bromodeoxyuridine immunocytochemistry. Ultrastructural observation showed that the swollen mitochondria, dilatation and vesiculation of rER and proliferation of peroxisome-like granules appeared within the cytoplasm of HepA2 cells following drug treatment. Using enzyme cytochemistry, both peroxidase and acid phosphatase activities but not catalase activity were localised in these peroxisome-like granules. Therefore, these results suggested that (a) 13- lapachone has a novel cytotoxic effect on human hepatoma cell; (2) B-lapachone induces the interruption of the cell cycle and unscheduled DNA synthesis in HepA2 cells; and (3) B-lapachone promotes the proliferation of peroxisome-like granules containing peroxidase and acid phosphatase activities without evidence of catalase activity in hepatoma cell line. | es |
dc.format | application/pdf | es |
dc.format.extent | 9 | es |
dc.language | eng | es |
dc.publisher | F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Hepatoma cells | es |
dc.subject | B-lapachone | es |
dc.subject | Cell death | es |
dc.subject | Electron microsocpy | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina | es |
dc.title | β-lapachone induced cell death in human hepatoma (HepA2) cells | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.13, nº 1 (1998) |
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O-lapachone induced cell death in human hepatoma (HepA2) cells.pdf | 5,42 MB | Adobe PDF | Visualizar/Abrir |
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