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Título: | Sialoglycoconjugate dimorphism of the mouse submandibular gland acinar cells. Ultrastructural evidence by lectin-protein A-gold probes and sialidase digestion |
Fecha de publicación: | 1998 |
Editorial: | F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología |
Cita bibliográfica: | Histology and histopathology, Vol. 13, nº 1 (1998) |
ISSN: | 1699-5848 0213-3911 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina |
Palabras clave: | Lectin-gold techniques Sialidase Submandibular gland Male and female mouse |
Resumen: | An ultrastructural analysis of lectin receptors on th e submandibular glands fro m mice of both sexes was performed utilizing horseradish pero xidase-labell ed lectins in conjunction with antiperox idase antibody and protein A-gold . Both qu alitati ve and quantit ati ve sexrelated differe nces in terminal suga r expression within secre tory gran ul es were detected. Following sia lidase digestion. also suhtenninal acceptor suga rs for terminal siali c ac ids, proved to be differentiall y ex pressed in the submandibular g land s of mal es and fema le s. Heteroge neo us distributi on of sialoglycoconjugates characteri zed bv the terminal disaccharide sialic acid-/3- galactose was f~ llIndt o occur in female ac inar ce lls. Also DBA reacti ve sites indicating the presence of terminal CI.-N-ace tylgalactosamin e discriminated betwee n male and female ac in ar sec re tor y glycoco nju ga tes. This difference was emphasized by sialidase pretreatment that ev idenced a marked occurrence of sialic acid subtended to u- N-ace tvlga lac tosamine in males in contrast to a modest pr es-e ~ce in fe males. The different sial ylation pa tt e rn s of acinar cell sec reto ry prod uc ts, pro bab ly related to a different exp ressio n of 0- and N-linked sia logl ycoconjugates, g iv e in sig ht into th e sex ual dimorphism of the mouse submandibular gland known until rece ntl y for the convoluted granular tubules. |
Autor/es principal/es: | Menghi, G. Bondi., A. M. Marchetti, L. Gabrielli, M. G. Materazzi, G. |
URI: | http://hdl.handle.net/10201/92525 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 10 |
Derechos: | info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 International |
Aparece en las colecciones: | Vol.13, nº 1 (1998) |
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Sialoglycoconjugate dimorphism of the mouse submandibular gland acinar cells..pdf | 5,16 MB | Adobe PDF | Visualizar/Abrir |
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