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dc.contributor.authorWehrl, Hans F.-
dc.contributor.authorBezrukov, Ilja-
dc.contributor.authorWiehr, Stefan-
dc.contributor.authorLehnhoff, Mareike-
dc.contributor.authorFuchs, Kerstin-
dc.contributor.authorMannheim, Julia G.-
dc.contributor.authorQuintanilla-Martine, Leticia-
dc.contributor.authorKohlhofer, Ursula-
dc.contributor.authorKneilling, Manfred-
dc.contributor.authorPichler, Bernd J.-
dc.contributor.authorSauter, Alexander W.-
dc.date.accessioned2020-04-21T12:32:57Z-
dc.date.available2020-04-21T12:32:57Z-
dc.date.issued2015-
dc.identifier.citationHistology and Histopathology, Vol. 30, n.º 5 (2015)es_ES
dc.identifier.issn1699-5848-
dc.identifier.issn0213-3911-
dc.identifier.urihttp://hdl.handle.net/10201/90174-
dc.description.abstractEspecially for neuroscience and the development of new biomarkers, a direct correlation between in vivo imaging and histology is essential. However, this comparison is hampered by deformation and shrinkage of tissue samples caused by fixation, dehydration and paraffin embedding. We used magnetic resonance (MR) imaging and computed tomography (CT) imaging to analyze the degree of shrinkage on murine brains for various fixatives. After in vivo imaging using 7 T MRI, animals were sacrificed and the brains were dissected and immediately placed in different fixatives, respectively: zinc-based fixative, neutral buffered formalin (NBF), paraformaldehyde (PFA), Bouin-Holland fixative and paraformaldehyde-lysine-periodate (PLP). The degree of shrinkage based on mouse brain volumes, radiodensity in Hounsfield units (HU), as well as non-linear deformations were obtained. The highest degree of shrinkage was observed for PLP (68.1%, P<0.001), followed by PFA (60.2%, P<0.001) and NBF (58.6%, P<0.001). The zinc-based fixative revealed a low shrinkage with only 33.5% (P<0.001). Compared to NBF, the zinc-based fixative shows a slightly higher degree of deformations, but is still more homogenous than PFA. Tissue shrinkage can be monitored non-invasively with CT and MR. Zinc-based fixative causes the smallest degree of brain shrinkage and only small deformations and is therefore recommended for in vivo ex vivo comparison studies.es_ES
dc.formatapplication/pdfes_ES
dc.format.extent13es_ES
dc.languageenges_ES
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histologíaes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectImaginges_ES
dc.subjectFixationes_ES
dc.subjectParaffines_ES
dc.subjectShrinkagees_ES
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes_ES
dc.titleAssessment of murine brain tissue shrinkage caused by different histological fixatives using magnetic resonance and computed tomography imaginges_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doihttps://doi.org/10.14670/HH-30.601-
Aparece en las colecciones:Vol.30, nº5 (2015)

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