Por favor, use este identificador para citar o enlazar este ítem: https://doi.org/10.14670/HH-30.589

Título: 3D Immunofluorescence analysis of early thymic morphogenesis and medulla development
Fecha de publicación: 2015
Editorial: F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
Cita bibliográfica: Histology and Histopathology, Vol. 30, n.º 5 (2015)
ISSN: 1699-5848
Materias relacionadas: CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología
Palabras clave: Thymus organogenesis
Thymic epithelium
Branching morphogenesis
Resumen: The thymus represents an epithelial microenvironment specialized in the generation of Tcells. The mechanisms or signals that determine the initial differentiation of the two well distinguished histological compartments of the thymus, cortex and medulla, remain unknown. Here, we report a threedimensional analysis of the distribution of some established thymic epithelial markers in relation to thymic anatomical development during the first steps of thymus organogenesis. In the thymic primordium, initial lumen is lined by claudin (Cld)3/4+K5+ cells, after thymus growth and lobulation they form a continuous branched structure that increases its length and branching degree. Within it, the presence of luminal structures can be distinguished, even at E13.5. The medullary marker mouse thymic stroma 10 (MTS10) is upregulated in these Cld3/4+ lumen forming cells in a proximal-distal sequence. This structural organisation is histologically similar to that described in other epithelial organs undergoing a branching morphogenesis process. These results indicate that the thymic medulla can be evidenced as a continuous branched structure from early stages and suggest a thymic developmental program based on or containing elements of a branching morphogenesis program modified by the presence of lymphoid cells, in which medullary epithelial cell commitment is initially determined by lumen formation.
Autor/es principal/es: Muñoz, Juan José
Cejalvo, Teresa
Tobajas, Esther
Fanlo, Lucía
Cortés, Alfonso
Zapata, Agustín Gregorio
URI: http://hdl.handle.net/10201/90171
DOI: https://doi.org/10.14670/HH-30.589
Tipo de documento: info:eu-repo/semantics/article
Número páginas / Extensión: 11
Derechos: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 International
Aparece en las colecciones:Vol.30, nº5 (2015)

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