Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/74975

Title: Characterization of rat testicular teratoma and its derived cell lines, with particular reference to possible mesenchymal differentiations
Issue Date: 2014
Publisher: F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
Citation: Histology and Histopathology, vol. 29, nº 9, (2014)
ISSN: 1699-5848
0213-3911
Related subjects: CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología
Keywords: Cell line
Mesenchymal differentiation
Stem cell
Rat testis
Abstract: The original tumor, 4 cm in diameter, was found in the left testis of a 2-month old SD rat. The tumor consisted of well-differentiated, mature tissues such as bone, cartilage, adipose tissue, smooth and skeletal muscles, skin, hair, glands (salivary, sebaceous, apocrine and pancreatic exocrine glands) and trachea, as well as nerve tissues. The tumor was diagnosed as a mature type of teratoma, a rare in rat testis. Cloned cell lines (named TSD-B4S and TSD-F9R) were established from the tumor; cellular properties of these cell lines were similar to each other; basically, their cultured cells exhibited vimentin-positive mesenchymal nature with occasional cells reacting to α-smooth muscle actin, glial fibrillary acidic protein and CD163 (a macrophage marker). The cell lines showed tumorigenicity when inoculated into nude mice, being composed of immature mesenchymal cells arranged mainly in a sheet. In TSDB4S cells treated with differentiation factors, we demonstrated mesenchymal differentiations towards adipogenic, osteogenic and myofibrogenic cells. The cell line (TSD-B4S) would become a useful tool for studies on stem cell differentiation, because the teratoma arises from primordial germ cells like embryonic stem cells.
Primary author: Yamate, Jyoji
Gamou, Katsuhiro
Izawa-Ogata, Keiko
Kotera, Takashi
Izawa, Takeshi
Takenaka, Shigeo
Sawamoto, Osamu
Kuwamura, Mitsuru
URI: http://hdl.handle.net/10201/74975
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 16
Rights: info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 International
Appears in Collections:Vol.29, nº 9 (2014)

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