Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/68699

Title: Morphological and immunohistochemical evaluation of ganglion cysts. Cross-sectional study of 354 cases
Issue Date: 2014
Publisher: F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
Citation: Histology and Histopathology, vol. 29, nº 5, (2014)
ISSN: 1699-5848
0213-3911
Related subjects: CDU::6 - Ciencias aplicadas::61 - Medicina
Keywords: Ganglion cyst
Soft tissue
Multifunctional mesenchymal cells
Abstract: The aim of this study was to characterize the morphology and immunophenotype of ganglion cysts (GCs) and explore their histogenetic origin. Material and Methods: A cross-sectional morphological and immunohistochemical study of 354 GCs used the following antibody panel: vimentin, specific actin, ß- actin, smooth-muscle actin, smoothelin, h-caldesmon, ß- catenin, desmin, calponin, podoplanin, keratins 5/6, Ecadherin, cyclooxygenase 2 (COX-2), lysozyme, CD10, CD31, CD33, CD34, CD68, Ki-67, and PCNA. Doubleblind semi-quantitative analyses were conducted to evaluate the immunopositivity on a 4-point scale. Samples from 10 synovial membranes and 10 scapholunate ligaments were compared. GCs showed a hyalinized wall with mesenchymal spindle cells and were intensely positive for vimentin, actins, h-caldesmon, calponin in all cases and for podoplanin in 53% of cases, suggesting features of early muscle differentiation, without ruling out a myofibroblastic origin. Focal cavity lining of nonsynovial flat or raised cells (CD34/CD31/CD10/Ecadherin-negative and podoplanin-positive in 34% of cases) was detected in 93% of cases, showing differential expression with synovial membrane and scapholunate ligament cells. Nuclear positivity for proliferative markers was observed in GC wall cells (258.1±255; 1019.3±316 positive cells/mm2, Ki-67 and PCNA, respectively) but positivity for these markers was significantly lower (p<0.001 Mann Whitney U-test) in scapholunate ligament samples. Conclusion: In this first immunohistochemical study of GCs, focal cellular lining of the cavity was observed in almost all cases, and the immunophenotype was identical to that of GC wall cells. These cells are immunohistochemically different from synoviocytes and scapholunate ligament cells and show characteristics of myofibroblasts or mesenchymal cells undergoing early muscle differentiation.
Primary author: O’Valle, Francisco
Hernández-Cortés, Pedro
Aneiros Fernández, José
Caba Molina, Mercedes
Gómez-Morales, Mercedes
Cámara, Miguel
Payá, Jorge A.
Aguilar, David
Del Moral, Raimundo G.
Aneiros, Jose
URI: http://hdl.handle.net/10201/68699
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 7
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.29, nº 5 (2014)

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