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Título: Proteomic analysis in usual and nonspecific interstitial pneumonia
Fecha de publicación: 2014
Editorial: F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
Cita bibliográfica: Histology and Histopathology, vol. 29, nº 3 (2014)
ISSN: 1699-5848
0213-3911
Materias relacionadas: CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología
Palabras clave: Proteomics
Usual interstitial pneumonia
Resumen: Differentiating nonspecific interstitial pneumonia (NSIP) from usual interstitial pneumonia (UIP) is important for the determination of both treatment and prognosis. Using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE), we examined 8 UIPs, 8 NSIPs, and 30 normal lung tissues. Comparisons with control in 2D-DIGE showed that (a) in UIP, nine protein spots were significantly upregulated and seven were significantly downregulated, (b) in NSIP, four protein spots were significantly upregulated and nine were significantly downregulated. The detected proteins were analyzed by MALDI-TOF mass spectrometry, allowing qualitative differences in vimentin subtypes to be characterized. One vimentin subtype was upregulated in UIP, while another one was downregulated in NSIP (vs. control). These different characteristics were partially supported by the results of Western blot analysis. Our immunohistochemistry revealed vimentin expression within fibroblasts (a) in fibroblastic foci in UIP and (b) in fibrotic alveolar walls in NSIP. Differences in vimentin subtypes may provide useful biomarkers for separating NSIP from UIP, alongside differences in histological characteristics.
Autor/es principal/es: Ohara, Ichiyo
Aida, Shinsuke
Shimazaki, Hideyuki
Kobayashi, Hideo
Tsuda, Hitoshi
Toda, Tosifusa
Nakanishi, Kuniaki
Tamai, Seiichi
URI: http://hdl.handle.net/10201/67123
Tipo de documento: info:eu-repo/semantics/article
Derechos: info:eu-repo/semantics/openAccess
Aparece en las colecciones:Vol.29, nº 3 (2014)

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