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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Bonetti, Maria Ida | - |
dc.contributor.author | Bacci, Stefano | - |
dc.contributor.author | Santosuosso, Michela | - |
dc.contributor.author | Mazzanti, Benedetta | - |
dc.contributor.author | Aldinucci, Alessandra | - |
dc.contributor.author | Ballerini, Clara | - |
dc.contributor.author | Guasti, Daniele | - |
dc.contributor.author | Calosi, Laura | - |
dc.contributor.author | Bosi, Alberto | - |
dc.contributor.author | Romagnoli, Paolo | - |
dc.date.accessioned | 2019-01-30T14:32:27Z | - |
dc.date.available | 2019-01-30T14:32:27Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Histology and Histopathology, vol. 29, nº 3 (2014) | es |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.uri | http://hdl.handle.net/10201/67103 | - |
dc.description.abstract | Dendritic cells and their precursors express PPAR-gamma, whose stimulation has inhibitory effects on the maturation and function of dendritic cells in vivo. Dendritic cells can differentiate in vitro from CD133+ progenitors; the influence of PPAR-gamma stimulation on this process is unknown. We have addressed the effect of PPAR-gamma agonist rosiglitazone, at a concentration as used in clinics, on the differentiation of dendritic cells from human CD133+ progenitors. Cells were harvested from cord blood by density gradient and immunomagnetic separation, and cultured for 18 days with fetal calf serum, cytokines and 1 µmol/L rosiglitazone. Analyses included flow cytometry, electron microscopy and mixed lymphocyte reaction. As expected, control cells generated without rosiglitazone were dendritic, expressed MHC-II, CD80, CD83 and CD86 and stimulated mixed reaction potently. A minority of cells expressed the Langerhans cell marker CD207/langerin, but none contained Birbeck granules. With rosiglitazone much fewer cells were generated; they were all dendritic, expressed differentiation and maturation-related antigens in higher percentage and were better stimulators of lymphocytes than those generated without the drug. The vast majority of cells expressed CD207/langerin and many contained Birbeck granules, i.e. were full-fledged Langerhans cells. We conclude that stimulation of PPAR-gamma, while negatively affecting the number of generated cells, promotes the maturation of human cord blood CD133 positive precursors into efficient, immunostimulating dendritic cells with a Langerhans cell phenotype. | es |
dc.format | application/pdf | es |
dc.format.extent | 10 | es |
dc.language | eng | es |
dc.publisher | F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Cell culture | es |
dc.subject | Electron microscopy | es |
dc.subject.other | CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología | es |
dc.title | Rosiglitazone promotes the differentiation of Langerhans cells and inhibits that of other dendritic cell types from CD133 positive hematopoietic precursors | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.29, nº 3 (2014) |
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Bonetti-29-323-332-2014.pdf | 3,27 MB | Adobe PDF | Visualizar/Abrir |
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