Por favor, use este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10201/63179
Twittear
Título: | Novel polyvinyl alcohol (PVA) based cryoprotection method that facilitates cutting frozen sections of decalcified human trabecular bone |
Fecha de publicación: | 2013 |
Editorial: | F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología |
Cita bibliográfica: | Histology and Histopathology, vol. 28, nº 12 (2013) |
ISSN: | 1699-5848 0213-3911 |
Materias relacionadas: | CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología |
Palabras clave: | Cryoprotection Cryomicrotomy |
Resumen: | Processing adult human trabecular bone to obtain tissue sections suitable for research or diagnostic purposes has always been challenging, particularly in the preparation of adult bone specimens for advanced immunohistochemistry applications. In contrast to the majority of soft tissues, decalcified bone samples perform poorly under standard paraffin embedding techniques and immunolabeling protocols fail frequently, due to the loss of protein antigenicity observed. We report on a new, PVA based infiltration method that avoids excessive heat exposure to tissue samples during embedding. The developed PVA based infiltration medium provides sufficient structural support to the heterogenic morphology and distinct architecture of subchondral trabecular bone and adjacent articular cartilage. Furthermore, the addition of bovine serum albumin (BSA) to this infiltration solution guaranteed safe attachment of cryosections to glass slides. The protocol allows the preparation of high quality sections of adult human trabecular bone tissues which can be used for both classical histochemical stains and for immunohistochemistry, since protein antigenicity is satisfactorily preserved. |
Autor/es principal/es: | Maly, I. Piotr Rahner, Christoph Nowakowski, Andrej Maria Müller-Gerbl, Magdalena |
URI: | http://hdl.handle.net/10201/63179 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 7 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.28, nº12 (2013) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Maly-28-1605-1611-2013.pdf | 6,27 MB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons