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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Ichikawa, Chisa | - |
dc.contributor.author | Izawa, Takeshi | - |
dc.contributor.author | Juniantito, Vetnizah | - |
dc.contributor.author | Tanaka, Miyuu | - |
dc.contributor.author | Hori, Mayuka | - |
dc.contributor.author | Tanaka, Katsuhiro | - |
dc.contributor.author | Takenaka, Shigeo | - |
dc.contributor.author | Kuwamura, Mitsuru | - |
dc.contributor.author | Yamate, Jyoji | - |
dc.date.accessioned | 2018-02-19T11:30:06Z | - |
dc.date.available | 2018-02-19T11:30:06Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Histology and Histopathology, vol. 28, nº 2, (2013) | es |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.uri | http://hdl.handle.net/10201/56236 | - |
dc.description.abstract | . A3 was generated as an antibody recognizing somatic stem cells in rat tissues. We investigated the distribution of A3-positive cells in developing rat hair follicles by immunolabeling. A3-positive cells began to be seen in the hair germ and peg in fetuses and neonates; the positive cells were epithelial cells above basal cells. Furthermore, A3-positive cells were seen in the outer root sheath adjacent to the bulge in mature hair follicles. Double immunofluorescence revealed that these A3- positive epithelial cells reacted to E-cadherin (for all epithelial elements) but not to CK15 (for basal cells/epithelial stem cells) or to nestin (for stem cells), indicating that A3-positive epithelial cells are suprabasal cells in the developing epidermic hair follicle. Additionally, spindle-shaped mesenchymal cells surrounding the hair peg and mature hair follicle reacted to A3; in double immunofluorescence, the A3-positive cells were located outside collagen type IV-positive glassy membrane, and reacted to vimentin (for mesenchmal cells), Thy-1 (for immature mesenchymal cells), CD34 (for stem cells) and nestin, but not to α- smooth muscle actin (for myofibroblasts); the positive cells were regarded as immature mesenchymal cells with stem cell nature in the connective tissue sheath of developing hair follicles. A3-positive epithelial and mesenchymal cells did not show proliferating activity. Collectively, it is considered that A3-positive cells seen in developing rat hair follicles may be quiescent postprogenitor cells with the potential to differentiate into either highly-differentiated epithelial or mesenchymal cells. A3 would become a useful antibody to know the kinetics of rat hair follicle-constituting cells. | es |
dc.format | application/pdf | es |
dc.format.extent | 12 | es |
dc.language | eng | es |
dc.publisher | F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Antibody | es |
dc.subject | Immunohistochemistry | es |
dc.subject | Rat hair follicle | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina | es |
dc.title | Rat hair follicle-constituting cells labeled by a newly-developed somatic stem cell-recognizing antibody: a possible marker of hair follicle development | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.28, nº 2 (2013) |
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Ichikawa-28-257-268-2013.pdf | 11,79 MB | Adobe PDF | Visualizar/Abrir |
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