Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/56014

Title: Expression pattern of the endoplasmic reticulum stress protein GP96 in monophasic and chronic relapsing form of experimental autoimmune encephalomyelitis in rats
Issue Date: 2013
Publisher: F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
Citation: Histology and histopathology, Vol. 28, n.º 1 (2013)
ISSN: 1699-5848
0213-3911
Related subjects: CDU::5 - Ciencias puras y naturales::57 - Biología::575 - Genética general. Citogenética general. Inmunogenética. Evolución. Filogenia
Keywords: Endoplasmic reticulum resident heat shock protein
GP96
Abstract: Gp96 is the endoplasmic reticulum (ER)- resident molecular chaperone, which is involved in the correction of unfolded proteins, in the activation of proteasome-dependent ER-associated degradation of the misfolded proteins, and in activation of the protein translation that modulates polypeptide traffic into the ER. Furthermore, owing to its peptide chaperone capacity and ability to interact with professional antigenpresenting cells, as well as with growth factors, integrins and Toll-like receptors, it is also endowed with crucial immunological functions acting as a “danger signal” to the innate and adaptive immunity. Considering these properties, in the present study the tissue expression of gp96 was examined during the monophasic and chronic relapsing form of experimental autoimmune encephalomyelitis (CR-EAE), induced in genetically susceptible DA rats by subcutaneous injection of myelin basic protein (MBP) or bovine brain homogenate in complete Freund's adjuvant (CFA). Immunohistochemical analyses were done in periods of attacks and remissions of EAE, and the results were compared with findings in intact rats and those treated only with CFA. The data revealed that the constitutive gp96 expression, found in several neurons and glial cells in the brain and spinal cord of intact animals, significantly diminished during the attacks of CR-EAE. On the contrary, the remission of disease was followed by high upregulation of gp96, mainly in the oligodendrocytes within the white matter, in the neurons of the hippocampal area, as well as in the motoneurons of lumbar spinal cord, suggesting that gp96 might be involved in proteostasis and immune-related pathways linked with the reparative processes in the CNS.
Primary author: Jakovac, Hrvoje
Grebić, Damir
Barac-Latas, Vesna
Mrakovčić-Šutić, Ines
Radošević-Stašić, Biserka
URI: http://hdl.handle.net/10201/56014
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 18
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.28, nº 1 (2013)

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