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Título: | Domestic microwave processing for rapid immunohistochemical diagnosis of bovine rabies |
Fecha de publicación: | 2012 |
Editorial: | F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología |
Cita bibliográfica: | Histology and histopathology, Vol. 27, nº 9 (2012) |
ISSN: | 1699-5848 0213-3911 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca::636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos |
Palabras clave: | Cattle Histopathology |
Resumen: | The present study describes the use of a microwave processing protocol for the rapid histopathological and immunohistochemical diagnosis of bovine rabies. Immunohistochemistry has been used for rabies diagnosis in formalin-fixed tissue with satisfactory results, although the time to diagnosis is considerably longer than that with direct immunofluorescence. The protocol provided a provisory histopathological rabies diagnosis in approximately three and half hours and the immunohistochemical diagnosis was available after six hours. The protocol achieved 100% correlation with direct immunofluorescence and is a promising method, particularly in situations in which only material in formalin is available for diagnosis or when the refrigeration or transportation of biological material is difficult. |
Autor/es principal/es: | Abreu, Camila C. Nakayama, Priscilla A. Nogueira, Clayton I. Mesquita, Leonardo P. Lopes, Priscila F.R. Varaschin, Mary S. Seixas, Josilene do N. Ferreira, Enio Bezerra Jr, Pedro S. |
URI: | http://hdl.handle.net/10201/54146 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 4 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.27, nº 9 (2012) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Abreu-27-1227-1230-2012.pdf | 3,22 MB | Adobe PDF | Visualizar/Abrir |
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