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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | de la Fuente, Isabel | - |
dc.contributor.author | Alcalde, Ignacio | - |
dc.contributor.author | Gamboa, Olga L. | - |
dc.contributor.author | Garrosa, Manuel | - |
dc.contributor.author | Gayoso, Manuel J. | - |
dc.date.accessioned | 2017-03-08T16:35:24Z | - |
dc.date.available | 2017-03-08T16:35:24Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Histology and histopathology, Vol. 27, nº 1 (2012) | es |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.uri | http://hdl.handle.net/10201/52379 | - |
dc.description.abstract | Schwann cells (SCs) are basic elements for cell therapy and tissue engineering in the central and peripheral nervous system. Therefore, the development of a reliable method to obtain SC cultures is required. For possible therapeutic applications the cultures need to produce a sufficiently large number of SCs with a high level of purity in a relatively short period of time. To increase SC yield and purity we pre-degenerated pieces of 1-2 mm of adult rabbit sciatic nerves by incubating them for seven days in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum, penicillin/streptomycin and NRG1-ß1. Following pre-degeneration the nerve pieces were dissociated and then cultured for 6 or 15 days in the same culture medium. After 6 days of culture we obtained around 9.5x103 cells/mg with approximately 94% SCs (S-100 positive) purity. After 15 days of culture the yield was about 80x103 cells/mg and the purity was approximately 75%. Pre-degeneration and subsequent culture of small pieces of adult nerve with NRG1-ß1 supplemented medium increased the number of SCs and restricted the overgrowth of fibroblast-like cells | es |
dc.format | application/pdf | es |
dc.format.extent | 8 | es |
dc.language | eng | es |
dc.publisher | F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Schwann cell | es |
dc.subject | Nerve pre-degeneration | es |
dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología::616.8 - Neurología. Neuropatología. Sistema nervioso | es |
dc.title | A method for obtaining Schwann cell cultures from adult rabbit nerve based on “in vitro” pre-degeneration and neuregulin treatment | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.27, nº 1 (2012) |
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Fichero | Descripción | Tamaño | Formato | |
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de-la-Fuente-27-95-102-2012.pdf | 4,41 MB | Adobe PDF | Visualizar/Abrir |
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