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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Petri, M.K. | - |
dc.contributor.author | Stenzinger, A. | - |
dc.contributor.author | Kuchelmeister, K. | - |
dc.contributor.author | Nestler, U. | - |
dc.contributor.author | Paradowska, A. | - |
dc.contributor.author | Steger, K. | - |
dc.contributor.author | Brobeil, A. | - |
dc.contributor.author | Viard, M. | - |
dc.contributor.author | Wimmer, M. | - |
dc.date.accessioned | 2016-05-03T16:34:40Z | - |
dc.date.available | 2016-05-03T16:34:40Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.uri | http://hdl.handle.net/10201/49529 | - |
dc.description.abstract | Glioblastoma multiforme (GBM) is the most common and most malignant primary brain tumour. Protein tyrosine phosphatase interacting protein 51 (PTPIP51) is an interaction partner of 14-3-3ß, which correlates with the grade of malignancy in gliomas. In this study PTPIP51 and its interacting partners 14-3-3ß, PTP1B, c-Src, Raf-1 as well as EGFR were investigated in human glioblastoma. Twenty glioblastoma samples were analyzed on transcriptional and translational level by immunohisto-chemistry, in situ hybridization and RT-PCR. To compare PTPIP51 expression in gliomas of different malignancies, quantitative RT-PCR for grade II astrocytoma and GBM samples was employed. Additionally, we analyzed the correlation between PTPIP51 and 14-3-3ß transcription, and checked for in situ interaction between PTPIP51 and 14-3-3ß and PTP1B, respectively. PTPIP51 and 14-3-3ß mRNA showed a tumour grade dependent upregulation in gliomas. Glioblastoma cells displayed a strong immunoreaction of PTPIP51, which co-localized with 14-3-3ß and PTP1B. The duolink proximity ligation assay corroborated a direct in situ interaction of PTPIP51 with both proteins, known to interact with PTPIP51 in vitro. The in vitro interacting partners Raf-1 and c-Src showed a partial co-localization. Besides, immune cells located in capillaries or infiltrating the tumour tissue and endothelial cells of pseudoglomerular vessels revealed a high PTPIP51 expression. The upregulation of PTPIP51 and its connection with the EGFR/MAPK pathway by 14-3-3ß via Raf-1 and by PTP1B via c-Src, argue for a functional role of PTPIP51 in the pathogenesis of human glioblastoma. | es |
dc.format | application/pdf | es |
dc.format.extent | 13 | es |
dc.language | eng | es |
dc.publisher | F. Hernández y J.F. Madrid. Murcia: Universidad de Murcia, Departamento de Biología Celular e Histología. | es |
dc.relation.ispartof | Histology and histopathology, Vol. 26, nº12 (2011) | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Glioblastoma | es |
dc.subject | PTPIP51 | es |
dc.subject.other | 616 - Patología. Medicina clínica. Oncología | es |
dc.title | PTPIP51, a positive modulator of the MAPK/Erk pathway, is upregulated in glioblastoma and interacts with 14-3-3ß and PTP1B in situ | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.26,nº12 (2011) |
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Petri-26-1531-1543-2011.pdf | 7,24 MB | Adobe PDF | Visualizar/Abrir |
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