Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/49445

Title: Site differences of Toll-like receptor expression in the mucous epithelium of rat small intestine
Issue Date: 2011
Publisher: F. Hernández y J.F. Madrid. Murcia: Universidad de Murcia, Departamento de Biología Celular e Histología.
ISSN: 1699-5848
0213-3911
Related subjects: 61 - Medicina
Keywords: Epithelium
Immunohistochemistry
Abstract: Toll-like receptors (TLRs) are known to recognize pathogen-associated molecular patterns and might function as receptors to detect microbes. In this study, the distribution of TLR-2, -4 and -9 were immunohistochemically investigated in the rat small intestine. As a result, TLR-2 was detected in the striated borders of villous columnar epithelial cells throughout the small intestine, except for the apices of a small number of intestinal villi. TLR-4 and -9 were detected in the striated borders of the villous columnar epithelial cells only in the duodenum. TLR-4-immunopositive minute granules were found in the apical cytoplasms of epithelial cells, subepithelial spaces and blood capillary lumina. TLR-2 and -4 were detected in the striated borders of undifferentiated epithelial cells and in the luminal substances of the intestinal crypts throughout the small intestine, but TLR-9 was not detected in the crypts throughout the small intestine. Only TLR-4 was detected in the secretory granules of Paneth cells in both the jejunal and ileal intestinal crypts. These findings suggest that duodenal TLRs might monitor indigenous bacteria proliferation in the upper alimentary tract, that TLR-2 might also monitor the proliferation of colonized indigenous bacteria throughout the small intestine, that the lack of TLR-2 at the villous apices might contribute to the settlement of indigenous bacteria, and that TLR-2 and -4 are secreted from intestinal crypts.
Primary author: Mantani, Youhei
Kamezaki, Aosa
Udayanga, Kankanam Gamage Sanath
Takahara, Ei-ichirou
Qi, Wang-Mei
Kawano, Junichi
Yokoyama, Toshifumi
Hoshi, Nobuhiko
Kitagawa, Hiroshi
Published in: Histology and histopathology, Vol. 26, nº10 (2011)
URI: http://hdl.handle.net/10201/49445
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 9
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol.26,nº10 (2011)

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