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Título: | Inflammatory cytokine and chemokine expression in sympathetic ophthalmia: a pilot study |
Fecha de publicación: | 2011 |
Editorial: | F. Hernández y J.F. Madrid. Murcia: Universidad de Murcia, Departamento de Biología Celular e Histología. |
ISSN: | 1699-5848 0213-3911 |
Materias relacionadas: | 616 - Patología. Medicina clínica. Oncología |
Palabras clave: | Chemokine Cytokines |
Resumen: | Sympathetic ophthalmia is a bilateral uveitis that develops after penetrating injury to one eye. This study aimed to identify the inflammatory cellular sub-phenotypes and expression of pertinent inflammatory cytokines/chemokines in sympathetic ophthalmia (SO). Dalen-Fuchs nodules (DFN), granulomas, and non-granulomatous foci of inflammation were micro-dissected from 15 cases. RNA was extracted, and quantitative PCR was performed to measure IL-17, IL-18, IL-23, IFN-γ, CCL19, CXCL11, CCL17, and CCL22 transcripts. Immunohistochemical methods were used to characterize CD3, CD4, CD8, CD20, CD68, and CD163 expression. Non-granulomatous lymphocytes were predominantly CD3-positive and expressed more IFN-γ than cells within granulomas, consistent with Th1 cells. In contrast, granulomas and DFN contained mainly CD68+, CD163+/- and expressed more IL-17, IL-18, IL-23, CCL19, and CXCL11 than non-granulomatous cells. Our data indicate for the first time that M1 macrophages are the predominant inflammatory cells within granulomas and DFN of SO. We further observed high levels of IL-17 within granulomas and the presence of Th1 and M1 cells. |
Autor/es principal/es: | Furusato, Emiko Shen, DeFen Cao, Xiaoguang Furusato, Bungo Nussenblat, Robert B. Rushing, Elisabeth J. Chan, Chi-Chao |
Forma parte de: | histology and histopathology, vol. 26, nº9 (2011) |
URI: | http://hdl.handle.net/10201/49385 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 7 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.26, nº9 (2011) |
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Fichero | Descripción | Tamaño | Formato | |
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Furusato-26-1145-1151-2011.pdf | 726,19 kB | Adobe PDF | Visualizar/Abrir |
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