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Título: | A role for mammalian target of rapamycin -mTOR- pathway in non alcoholic steatohepatitis related-cirrhosis |
Fecha de publicación: | 2010 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | 616 - Patología. Medicina clínica. Oncología |
Palabras clave: | Cirrhosis Non alcoholic steatohepatitis Gene expression profile |
Resumen: | Summary. Non-alcoholic fatty liver disease (NAFLD) encompasses the whole spectrum of steatosis, nonalcoholic steatohepatitis (NASH), and NASH-related cirrhosis (NASH/Cir). Although molecular advances have been made in this field, the pathogenesis of NAFLD is not completely understood. The gene expression profiling associated to NASH/Cir was assessed, in an attempt to better characterize the pathways involved in its etiopathogenesis. Methods: In the first step, we used cDNA microarray to evaluate the gene expression profiles in normal liver (n=3) and NASH/Cir samples (n=3) by GeneSifter™ analysis to identify differentially expressed genes and biological pathways. Second, tissue microarray was used to determine immunohistochemical expression of phosphorylated mTOR and 4E-BP1 in 11 normal liver samples, 10 NASH/Cir samples and in 37 samples of cirrhosis of other etiologies to further explore the involvement of the mTOR pathway evidenced by the gene expression analysis. Results: 138 and 106 genes were, respectively, up and down regulated in NASH/Cir in comparison to normal liver. Among the 9 pathways identified as significantly modulated in NASH/Cir, the participation of the mTOR pathway was confirmed, since expression of cytoplasmic and membrane phosphomTOR were higher in NASH/Cir in comparison to cirrhosis of other etiologies and to normal liver. Conclusions: Recent findings have suggested a role for the cellular “nutrient sensor” mTOR in NAFLD and the present study corroborates the participation of this pathway in NASH/Cir. Phospho-mTOR evaluation might be of clinical utility as a potential marker for identification of NASH/Cir in cases mistakenly considered as cryptogenic cirrhosis owing to paucity of clinical data. |
Autor/es principal/es: | Kubrusly, Márcia Saldanha Corrêa-Giannella, Maria Lúcia Bellodi-Privato, Marta de Sá, Sandra Valéria Cauduro Soares, Iberê Wakamatsu, Alda Avancini Ferreira Alves, Venâncio Giannella-Neto, Daniel Bacchella, Telesforo Cerqueira Cesar Machado, Marcel Carneiro D’Albuquerque, Luiz Augusto Pinto Marques Souza de Oliveira, Claudia |
Forma parte de: | Histology and histopathology (2010) vol. 25 |
URI: | http://hdl.handle.net/10201/46342 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 9 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.25, nº9 (2010) |
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A role for mammalian target of rapamycin mTOR pathway in non alcoholic steatohepatitis relatedcirrhosis.pdf | 781,5 kB | Adobe PDF | Visualizar/Abrir |
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